Results 41 - 45 of 45 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 2652 | 5' | -64.7 | NC_001491.2 | + | 147716 | 0.66 | 0.52016 |
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Target: 5'- -uGGCAGGguccggCCCGCgucaGCC-CCUACGg -3' miRNA: 3'- acCCGUCCa-----GGGCGg---CGGuGGGUGCg -5' |
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| 2652 | 5' | -64.7 | NC_001491.2 | + | 5181 | 0.66 | 0.523867 |
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Target: 5'- cGGGCAGGUCUcgguauCGCUgucgagggcgaccggGUCGgCCGCGUc -3' miRNA: 3'- aCCCGUCCAGG------GCGG---------------CGGUgGGUGCG- -5' |
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| 2652 | 5' | -64.7 | NC_001491.2 | + | 39445 | 0.66 | 0.527584 |
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Target: 5'- --cGCGGGcCCCaGUCGCCugggugcuggccCCCGCGCa -3' miRNA: 3'- accCGUCCaGGG-CGGCGGu-----------GGGUGCG- -5' |
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| 2652 | 5' | -64.7 | NC_001491.2 | + | 1363 | 0.66 | 0.538793 |
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Target: 5'- -cGGCAGGcacagCuCCGUgGCC-CCCAUGUg -3' miRNA: 3'- acCCGUCCa----G-GGCGgCGGuGGGUGCG- -5' |
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| 2652 | 5' | -64.7 | NC_001491.2 | + | 84768 | 0.65 | 0.547252 |
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Target: 5'- cGGGCagugccaAGGUCguaGCCGCUucuaccGCCC-CGCa -3' miRNA: 3'- aCCCG-------UCCAGgg-CGGCGG------UGGGuGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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