Results 41 - 45 of 45 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 2652 | 5' | -64.7 | NC_001491.2 | + | 136040 | 0.71 | 0.26614 |
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Target: 5'- aGGGCGGGaaccucugggaagggCUCGUCGCCAUgCGCGUu -3' miRNA: 3'- aCCCGUCCa--------------GGGCGGCGGUGgGUGCG- -5' |
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| 2652 | 5' | -64.7 | NC_001491.2 | + | 141754 | 0.68 | 0.382665 |
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Target: 5'- aGaGGCGGGUUCCGCUGggcaugcuUCACCUcgcaauGCGCg -3' miRNA: 3'- aC-CCGUCCAGGGCGGC--------GGUGGG------UGCG- -5' |
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| 2652 | 5' | -64.7 | NC_001491.2 | + | 144489 | 0.66 | 0.510938 |
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Target: 5'- gGGcGCAGaaaCCGCCGUCgACCCuucagGCGCa -3' miRNA: 3'- aCC-CGUCcagGGCGGCGG-UGGG-----UGCG- -5' |
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| 2652 | 5' | -64.7 | NC_001491.2 | + | 147716 | 0.66 | 0.52016 |
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Target: 5'- -uGGCAGGguccggCCCGCgucaGCC-CCUACGg -3' miRNA: 3'- acCCGUCCa-----GGGCGg---CGGuGGGUGCg -5' |
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| 2652 | 5' | -64.7 | NC_001491.2 | + | 148418 | 0.74 | 0.1835 |
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Target: 5'- gGGGC-GGUCaucaCGCuCGCCGUCCACGCc -3' miRNA: 3'- aCCCGuCCAGg---GCG-GCGGUGGGUGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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