Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 26526 | 5' | -58.6 | NC_005357.1 | + | 18264 | 0.66 | 0.458711 |
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Target: 5'- cGCCgaagCGCGcAGCCgucGAGGCGCUGg- -3' miRNA: 3'- -CGGaaggGCGC-UUGG---CUCCGCGACaa -5' |
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| 26526 | 5' | -58.6 | NC_005357.1 | + | 5317 | 0.66 | 0.448797 |
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Target: 5'- gGCCguaggCCGCGAGCaCGuGGCGCa--- -3' miRNA: 3'- -CGGaag--GGCGCUUG-GCuCCGCGacaa -5' |
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| 26526 | 5' | -58.6 | NC_005357.1 | + | 13045 | 0.66 | 0.439002 |
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Target: 5'- gGCCUaCCgCGcCGAGuCCGAGG-GCUGg- -3' miRNA: 3'- -CGGAaGG-GC-GCUU-GGCUCCgCGACaa -5' |
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| 26526 | 5' | -58.6 | NC_005357.1 | + | 31845 | 0.67 | 0.410372 |
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Target: 5'- gGCUgcgcgCCUGgGuacuacgcCCGAGGCGCUGUUc -3' miRNA: 3'- -CGGaa---GGGCgCuu------GGCUCCGCGACAA- -5' |
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| 26526 | 5' | -58.6 | NC_005357.1 | + | 18716 | 0.67 | 0.410372 |
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Target: 5'- gGCaCUggCCCGCG-ACCuGGGCGCUa-- -3' miRNA: 3'- -CG-GAa-GGGCGCuUGGcUCCGCGAcaa -5' |
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| 26526 | 5' | -58.6 | NC_005357.1 | + | 32115 | 0.67 | 0.401089 |
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Target: 5'- gGCCUggcgaaaCUgGCGAAguggcaccCCGAGGUGCUGg- -3' miRNA: 3'- -CGGAa------GGgCGCUU--------GGCUCCGCGACaa -5' |
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| 26526 | 5' | -58.6 | NC_005357.1 | + | 33441 | 0.68 | 0.356736 |
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Target: 5'- cGCC--CCCGCGcggcgaggaAAUCGAGGCGCUu-- -3' miRNA: 3'- -CGGaaGGGCGC---------UUGGCUCCGCGAcaa -5' |
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| 26526 | 5' | -58.6 | NC_005357.1 | + | 14991 | 0.68 | 0.323813 |
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Target: 5'- cGCCagggCCUGCGGccGCCG-GGCGCgGUg -3' miRNA: 3'- -CGGaa--GGGCGCU--UGGCuCCGCGaCAa -5' |
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| 26526 | 5' | -58.6 | NC_005357.1 | + | 6353 | 0.77 | 0.085976 |
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Target: 5'- cGCCcggCCCGaaGGCCGGGGCGCUGg- -3' miRNA: 3'- -CGGaa-GGGCgcUUGGCUCCGCGACaa -5' |
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| 26526 | 5' | -58.6 | NC_005357.1 | + | 10880 | 1.07 | 0.000456 |
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Target: 5'- cGCCUUCCCGCGAACCGAGGCGCUGUUu -3' miRNA: 3'- -CGGAAGGGCGCUUGGCUCCGCGACAA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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