Results 21 - 30 of 30 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 26527 | 3' | -60.6 | NC_005357.1 | + | 10332 | 0.69 | 0.214575 |
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Target: 5'- ----cAGGCCGuaGcCGUCGCUGCgGCGu -3' miRNA: 3'- cuacaUCCGGCggC-GCAGCGACG-CGC- -5' |
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| 26527 | 3' | -60.6 | NC_005357.1 | + | 27837 | 0.7 | 0.203624 |
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Target: 5'- cGcgGUGGcGCCgGCCGCGcCGCUGCuCa -3' miRNA: 3'- -CuaCAUC-CGG-CGGCGCaGCGACGcGc -5' |
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| 26527 | 3' | -60.6 | NC_005357.1 | + | 37550 | 0.7 | 0.183172 |
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Target: 5'- ----gAGGCCGCCGuCGccgCGCUGgGCa -3' miRNA: 3'- cuacaUCCGGCGGC-GCa--GCGACgCGc -5' |
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| 26527 | 3' | -60.6 | NC_005357.1 | + | 11153 | 0.71 | 0.169045 |
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Target: 5'- -cUGcUGGGCCGCCuGCGcaCGUUGCGUGc -3' miRNA: 3'- cuAC-AUCCGGCGG-CGCa-GCGACGCGC- -5' |
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| 26527 | 3' | -60.6 | NC_005357.1 | + | 891 | 0.72 | 0.143298 |
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Target: 5'- ----aGGGCCGCCGCGUUGUUccagaaguugaccGCGCu -3' miRNA: 3'- cuacaUCCGGCGGCGCAGCGA-------------CGCGc -5' |
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| 26527 | 3' | -60.6 | NC_005357.1 | + | 8140 | 0.74 | 0.094558 |
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Target: 5'- -uUGUGGGCgugcgggugucggCGCUGCGUCcCUGCGCGu -3' miRNA: 3'- cuACAUCCG-------------GCGGCGCAGcGACGCGC- -5' |
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| 26527 | 3' | -60.6 | NC_005357.1 | + | 16067 | 0.75 | 0.08964 |
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Target: 5'- --cGU-GGCCGCCGCG-CGCgGCGCc -3' miRNA: 3'- cuaCAuCCGGCGGCGCaGCGaCGCGc -5' |
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| 26527 | 3' | -60.6 | NC_005357.1 | + | 4348 | 0.75 | 0.087149 |
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Target: 5'- uGAUGcuGGCCGuuGCGUUGCcggUGCGCGu -3' miRNA: 3'- -CUACauCCGGCggCGCAGCG---ACGCGC- -5' |
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| 26527 | 3' | -60.6 | NC_005357.1 | + | 29403 | 0.81 | 0.031929 |
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Target: 5'- cGAUGacgAGGCCGUCGCcacugCGCUGCGCGg -3' miRNA: 3'- -CUACa--UCCGGCGGCGca---GCGACGCGC- -5' |
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| 26527 | 3' | -60.6 | NC_005357.1 | + | 9346 | 1.08 | 0.000242 |
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Target: 5'- gGAUGUAGGCCGCCGCGUCGCUGCGCGa -3' miRNA: 3'- -CUACAUCCGGCGGCGCAGCGACGCGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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