Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 2653 | 5' | -57.6 | NC_001491.2 | + | 82757 | 0.66 | 0.830745 |
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Target: 5'- cGACGCCGUAGcccuccaguacGAgcgcggcCUGGgGCUGCUg -3' miRNA: 3'- cCUGCGGCGUC-----------CUa------GACCgCGAUGAa -5' |
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| 2653 | 5' | -57.6 | NC_001491.2 | + | 128556 | 0.66 | 0.822295 |
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Target: 5'- cGGgGCCGCGGca-CUGGCGCUGg-- -3' miRNA: 3'- cCUgCGGCGUCcuaGACCGCGAUgaa -5' |
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| 2653 | 5' | -57.6 | NC_001491.2 | + | 35753 | 0.66 | 0.822295 |
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Target: 5'- cGACGCCG-AGGccgagcccCUGGUGCUGCc- -3' miRNA: 3'- cCUGCGGCgUCCua------GACCGCGAUGaa -5' |
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| 2653 | 5' | -57.6 | NC_001491.2 | + | 12493 | 0.66 | 0.822295 |
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Target: 5'- cGGGCGcCCGCGGG-UCcccggGGCGggGCg- -3' miRNA: 3'- -CCUGC-GGCGUCCuAGa----CCGCgaUGaa -5' |
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| 2653 | 5' | -57.6 | NC_001491.2 | + | 10431 | 0.67 | 0.749179 |
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Target: 5'- aGACGCCGCcggcGGAcgCaGaGCGCUACUg -3' miRNA: 3'- cCUGCGGCGu---CCUa-GaC-CGCGAUGAa -5' |
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| 2653 | 5' | -57.6 | NC_001491.2 | + | 69861 | 0.71 | 0.567576 |
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Target: 5'- cGGAUGgCGCGGGucgCUGGgGCUGgUUg -3' miRNA: 3'- -CCUGCgGCGUCCua-GACCgCGAUgAA- -5' |
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| 2653 | 5' | -57.6 | NC_001491.2 | + | 96961 | 1.07 | 0.002435 |
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Target: 5'- cGGACGCCGCAGGAUCUGGCGCUACUUc -3' miRNA: 3'- -CCUGCGGCGUCCUAGACCGCGAUGAA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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