miRNA: 3'- -CCGCUCgGUACUGGuuAAGcAGGUAG- -5'
miRNA: 3'- cCGCuCGGUACUGGUUa----AGCa--GGUAG- -5'
miRNA: 3'- -CCGcUCGGUACUGGUuaa--GCAGGUAG- -5'
miRNA: 3'- -CCGC--------UCGGUACUGGUUaagCaGGUAG- -5'
Results 21 - 24 of 24 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 26537 | 3' | -51.5 | NC_005357.1 | + | 30707 | 0.68 | 0.707394 |
|
Target: 5'- uGGCGGG-CAUGGCU--UUC-UCCAUCg -3' miRNA: 3'- -CCGCUCgGUACUGGuuAAGcAGGUAG- -5' |
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| 26537 | 3' | -51.5 | NC_005357.1 | + | 37687 | 0.7 | 0.627328 |
|
Target: 5'- cGCG-GCCGUGGCCGAgaacaUCGcggUCAUCa -3' miRNA: 3'- cCGCuCGGUACUGGUUa----AGCa--GGUAG- -5' |
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| 26537 | 3' | -51.5 | NC_005357.1 | + | 40682 | 0.76 | 0.320121 |
|
Target: 5'- aGGCcGGCCAUGACCGcccggUGUUCAUCu -3' miRNA: 3'- -CCGcUCGGUACUGGUuaa--GCAGGUAG- -5' |
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| 26537 | 3' | -51.5 | NC_005357.1 | + | 41709 | 0.66 | 0.823461 |
|
Target: 5'- aGGCGcucgacuuGGCCGUGGCCGAcaaGaUCAUCa -3' miRNA: 3'- -CCGC--------UCGGUACUGGUUaagCaGGUAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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