Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 26544 | 5' | -55.2 | NC_005357.1 | + | 14855 | 0.66 | 0.608785 |
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Target: 5'- cGGCCAGcgccgcacgcUGGGCGCCuuuAAGUUGc-- -3' miRNA: 3'- -UCGGUC----------ACCCGCGGcuuUUCAACcuu -5' |
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| 26544 | 5' | -55.2 | NC_005357.1 | + | 460 | 0.66 | 0.597369 |
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Target: 5'- cGGCCAGcuugcGGGCGCUGucgcGGUcGGAu -3' miRNA: 3'- -UCGGUCa----CCCGCGGCuuu-UCAaCCUu -5' |
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| 26544 | 5' | -55.2 | NC_005357.1 | + | 31199 | 0.66 | 0.597369 |
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Target: 5'- cGCUAGUGGGCGUgggCGGGAAcgUGGc- -3' miRNA: 3'- uCGGUCACCCGCG---GCUUUUcaACCuu -5' |
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| 26544 | 5' | -55.2 | NC_005357.1 | + | 15120 | 0.66 | 0.563351 |
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Target: 5'- cGCCuGUGGGCGUCGug-----GGAAg -3' miRNA: 3'- uCGGuCACCCGCGGCuuuucaaCCUU- -5' |
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| 26544 | 5' | -55.2 | NC_005357.1 | + | 1185 | 0.68 | 0.475843 |
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Target: 5'- cGCCGccgGGGUcgGCCGGGuuGUUGGAAu -3' miRNA: 3'- uCGGUca-CCCG--CGGCUUuuCAACCUU- -5' |
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| 26544 | 5' | -55.2 | NC_005357.1 | + | 36290 | 0.69 | 0.39563 |
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Target: 5'- cGGCgAGUGGGUGCUGA-----UGGAAg -3' miRNA: 3'- -UCGgUCACCCGCGGCUuuucaACCUU- -5' |
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| 26544 | 5' | -55.2 | NC_005357.1 | + | 34636 | 0.7 | 0.358928 |
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Target: 5'- cGCCuGcUGGGCGgCGAAGGGU-GGAc -3' miRNA: 3'- uCGGuC-ACCCGCgGCUUUUCAaCCUu -5' |
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| 26544 | 5' | -55.2 | NC_005357.1 | + | 19329 | 0.7 | 0.350133 |
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Target: 5'- cGCC-GUGGGCGCCGcc--GUUGGc- -3' miRNA: 3'- uCGGuCACCCGCGGCuuuuCAACCuu -5' |
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| 26544 | 5' | -55.2 | NC_005357.1 | + | 4188 | 1.06 | 0.000965 |
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Target: 5'- uAGCCAGUGGGCGCCGAAAAGUUGGAAu -3' miRNA: 3'- -UCGGUCACCCGCGGCUUUUCAACCUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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