Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 26548 | 5' | -57.2 | NC_005357.1 | + | 22648 | 0.66 | 0.558279 |
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Target: 5'- --gGGCCGGccuggcaaucAGUGaagaCCGC-CUCGUGGc -3' miRNA: 3'- gaaCCGGCC----------UCACa---GGCGcGAGUACC- -5' |
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| 26548 | 5' | -57.2 | NC_005357.1 | + | 18230 | 0.66 | 0.545351 |
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Target: 5'- uUUGGCagcgccuacGUGcUCGCGCUCAUGGa -3' miRNA: 3'- gAACCGgccu-----CACaGGCGCGAGUACC- -5' |
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| 26548 | 5' | -57.2 | NC_005357.1 | + | 18546 | 0.66 | 0.519802 |
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Target: 5'- -cUGGCCGaagucgaucacgucGGUGgaggCCGCGCUCGcGGu -3' miRNA: 3'- gaACCGGCc-------------UCACa---GGCGCGAGUaCC- -5' |
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| 26548 | 5' | -57.2 | NC_005357.1 | + | 29263 | 0.68 | 0.444409 |
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Target: 5'- --aGGCCGGccGGUGUCgGgGUggaCGUGGu -3' miRNA: 3'- gaaCCGGCC--UCACAGgCgCGa--GUACC- -5' |
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| 26548 | 5' | -57.2 | NC_005357.1 | + | 1197 | 0.68 | 0.415627 |
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Target: 5'- --cGGCCGGGuUGUuggaauaaCCGCcCUCAUGGc -3' miRNA: 3'- gaaCCGGCCUcACA--------GGCGcGAGUACC- -5' |
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| 26548 | 5' | -57.2 | NC_005357.1 | + | 8362 | 0.69 | 0.370289 |
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Target: 5'- --cGGCCGGGaUGUCCG-GCUCGa-- -3' miRNA: 3'- gaaCCGGCCUcACAGGCgCGAGUacc -5' |
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| 26548 | 5' | -57.2 | NC_005357.1 | + | 27848 | 0.69 | 0.360779 |
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Target: 5'- --cGGCCGcgccgcugcucaaGAacGUGUCCGCGCUCGccaucuUGGc -3' miRNA: 3'- gaaCCGGC-------------CU--CACAGGCGCGAGU------ACC- -5' |
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| 26548 | 5' | -57.2 | NC_005357.1 | + | 21143 | 0.78 | 0.095515 |
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Target: 5'- gCUUGGCCGccGUGUCCGCGC-CcUGGu -3' miRNA: 3'- -GAACCGGCcuCACAGGCGCGaGuACC- -5' |
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| 26548 | 5' | -57.2 | NC_005357.1 | + | 6645 | 1.1 | 0.000444 |
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Target: 5'- gCUUGGCCGGAGUGUCCGCGCUCAUGGu -3' miRNA: 3'- -GAACCGGCCUCACAGGCGCGAGUACC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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