Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 26549 | 3' | -50.1 | NC_005357.1 | + | 39364 | 0.66 | 0.88634 |
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Target: 5'- -gUGCGgcucGGGCGCU--UGACGUg- -3' miRNA: 3'- caACGUauu-CCCGCGAguACUGCAac -5' |
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| 26549 | 3' | -50.1 | NC_005357.1 | + | 30461 | 0.66 | 0.869918 |
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Target: 5'- aGUUGCcaGGGGGCGCcggUCAUcGCGUc- -3' miRNA: 3'- -CAACGuaUUCCCGCG---AGUAcUGCAac -5' |
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| 26549 | 3' | -50.1 | NC_005357.1 | + | 12138 | 0.67 | 0.833753 |
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Target: 5'- --cGCA--GGGGCGCUaCccGACGUUu -3' miRNA: 3'- caaCGUauUCCCGCGA-GuaCUGCAAc -5' |
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| 26549 | 3' | -50.1 | NC_005357.1 | + | 9692 | 0.72 | 0.541639 |
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Target: 5'- cUUG-GUGGGGGCGgUCAUGGCGUc- -3' miRNA: 3'- cAACgUAUUCCCGCgAGUACUGCAac -5' |
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| 26549 | 3' | -50.1 | NC_005357.1 | + | 38513 | 0.74 | 0.442726 |
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Target: 5'- --cGCAUGGGGGCGCggcCAUG-CGUg- -3' miRNA: 3'- caaCGUAUUCCCGCGa--GUACuGCAac -5' |
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| 26549 | 3' | -50.1 | NC_005357.1 | + | 7138 | 1.09 | 0.002013 |
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Target: 5'- gGUUGCAUAAGGGCGCUCAUGACGUUGa -3' miRNA: 3'- -CAACGUAUUCCCGCGAGUACUGCAAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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