Results 21 - 23 of 23 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
26555 | 5' | -55 | NC_005357.1 | + | 32858 | 0.75 | 0.21647 |
Target: 5'- cCGCAGACGGUcgGCgCCGagauUGUCGCgCCCg -3' miRNA: 3'- -GUGUUUGUCAa-CG-GGCg---ACAGCG-GGG- -5' |
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26555 | 5' | -55 | NC_005357.1 | + | 33413 | 0.76 | 0.178717 |
Target: 5'- gACGAACuGGUgcgccGCCUGCUGgaagCGCCCCc -3' miRNA: 3'- gUGUUUG-UCAa----CGGGCGACa---GCGGGG- -5' |
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26555 | 5' | -55 | NC_005357.1 | + | 15267 | 1.12 | 0.000431 |
Target: 5'- gCACAAACAGUUGCCCGCUGUCGCCCCa -3' miRNA: 3'- -GUGUUUGUCAACGGGCGACAGCGGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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