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miRNA: 3'- guUACCACGCugCG---UGCuGCCGUAUc -5'
miRNA: 3'- gUUACCA----------CGCUGCGUGCUGCCGuAUC- -5'
miRNA: 3'- -GUUACCACGCUGCGUGCUGCCGUAUC- -5'
Results 41 - 43 of 43 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio#
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P value |
| Predicted miRNA align pattern | |||||||
| 26557 | 5' | -53.2 | NC_005357.1 | + | 14960 | 0.74 | 0.329225 |
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Target: 5'- -cGUGGUGCGugGCggcGCGcUGGCAUAc -3' miRNA: 3'- guUACCACGCugCG---UGCuGCCGUAUc -5' |
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| 26557 | 5' | -53.2 | NC_005357.1 | + | 29951 | 0.84 | 0.074518 |
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Target: 5'- aGGUGGUgcuugucgucGCGGCGCACGACGGCcUGGa -3' miRNA: 3'- gUUACCA----------CGCUGCGUGCUGCCGuAUC- -5' |
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| 26557 | 5' | -53.2 | NC_005357.1 | + | 15773 | 1.09 | 0.001115 |
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Target: 5'- cCAAUGGUGCGACGCACGACGGCAUAGa -3' miRNA: 3'- -GUUACCACGCUGCGUGCUGCCGUAUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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