Results 41 - 43 of 43 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position![]() |
R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
26557 | 5' | -53.2 | NC_005357.1 | + | 36447 | 0.68 | 0.613205 |
Target: 5'- ------aGCGACGCA-GACGGUGUGGa -3' miRNA: 3'- guuaccaCGCUGCGUgCUGCCGUAUC- -5' |
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26557 | 5' | -53.2 | NC_005357.1 | + | 38751 | 0.71 | 0.449597 |
Target: 5'- -cGUGGUG-GAuuCGCACGAaGGCGUGGa -3' miRNA: 3'- guUACCACgCU--GCGUGCUgCCGUAUC- -5' |
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26557 | 5' | -53.2 | NC_005357.1 | + | 41219 | 0.68 | 0.601845 |
Target: 5'- gCAAUGG-GuCGAaaagGCGACGGCGUAGa -3' miRNA: 3'- -GUUACCaC-GCUgcg-UGCUGCCGUAUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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