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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 26558 | 5' | -56.4 | NC_005357.1 | + | 38886 | 0.66 | 0.60093 |
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Target: 5'- cGGUuGGCGggACUGCG-CGGuGUg- -3' miRNA: 3'- aCCAcCCGCaaUGGCGCaGCCuCAag -5' |
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| 26558 | 5' | -56.4 | NC_005357.1 | + | 17454 | 0.67 | 0.535292 |
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Target: 5'- cUGGUGGGUGgg--CGCGUC-GAGUa- -3' miRNA: 3'- -ACCACCCGCaaugGCGCAGcCUCAag -5' |
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| 26558 | 5' | -56.4 | NC_005357.1 | + | 18052 | 0.67 | 0.503393 |
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Target: 5'- uUGG-GGGCcUUACCgGCG-CGGGGUg- -3' miRNA: 3'- -ACCaCCCGcAAUGG-CGCaGCCUCAag -5' |
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| 26558 | 5' | -56.4 | NC_005357.1 | + | 4102 | 0.67 | 0.503393 |
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Target: 5'- aGGUGGGCGgcGCUGaauuCGUCGcGGUcgUCg -3' miRNA: 3'- aCCACCCGCaaUGGC----GCAGCcUCA--AG- -5' |
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| 26558 | 5' | -56.4 | NC_005357.1 | + | 10416 | 0.68 | 0.452196 |
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Target: 5'- -cGUGGGCGUccAgCGUGUCGaGGGUUUc -3' miRNA: 3'- acCACCCGCAa-UgGCGCAGC-CUCAAG- -5' |
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| 26558 | 5' | -56.4 | NC_005357.1 | + | 19332 | 0.68 | 0.432523 |
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Target: 5'- -cGUGGGCGccGCCguugGCGUCGGGGc-- -3' miRNA: 3'- acCACCCGCaaUGG----CGCAGCCUCaag -5' |
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| 26558 | 5' | -56.4 | NC_005357.1 | + | 16165 | 1.1 | 0.000468 |
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Target: 5'- gUGGUGGGCGUUACCGCGUCGGAGUUCa -3' miRNA: 3'- -ACCACCCGCAAUGGCGCAGCCUCAAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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