Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio #
|
P value |
| Predicted miRNA align pattern | |||||||
| 26559 | 3' | -50.3 | NC_005357.1 | + | 9832 | 0.66 | 0.871235 |
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Target: 5'- --uUGACgCGgG-CCAGCUUGGUCACa -3' miRNA: 3'- uuuAUUGgGCgUgGGUUGAGCUAGUG- -5' |
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| 26559 | 3' | -50.3 | NC_005357.1 | + | 41555 | 0.66 | 0.844649 |
|
Target: 5'- --cUGGCCCuGCugUuCAACUCGGcUCGCa -3' miRNA: 3'- uuuAUUGGG-CGugG-GUUGAGCU-AGUG- -5' |
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| 26559 | 3' | -50.3 | NC_005357.1 | + | 10717 | 0.66 | 0.844649 |
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Target: 5'- ---aAGCCCGCGCgCGGCgggcUGGUCAg -3' miRNA: 3'- uuuaUUGGGCGUGgGUUGa---GCUAGUg -5' |
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| 26559 | 3' | -50.3 | NC_005357.1 | + | 42015 | 0.66 | 0.862645 |
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Target: 5'- -cGUGGCCCGCgacgugcuGCUCAACagUCGcgCGCu -3' miRNA: 3'- uuUAUUGGGCG--------UGGGUUG--AGCuaGUG- -5' |
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| 26559 | 3' | -50.3 | NC_005357.1 | + | 40870 | 0.66 | 0.862645 |
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Target: 5'- aGGAUAugCC-CAUCCAGC-CGGUCGa -3' miRNA: 3'- -UUUAUugGGcGUGGGUUGaGCUAGUg -5' |
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| 26559 | 3' | -50.3 | NC_005357.1 | + | 28489 | 0.66 | 0.862645 |
|
Target: 5'- cGAgcAUCCGCucggcauuGCCgAACUCGGUCAUc -3' miRNA: 3'- uUUauUGGGCG--------UGGgUUGAGCUAGUG- -5' |
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| 26559 | 3' | -50.3 | NC_005357.1 | + | 22116 | 0.66 | 0.887555 |
|
Target: 5'- ---cAGCCCGCAUCgCAAC-CGGcCGCc -3' miRNA: 3'- uuuaUUGGGCGUGG-GUUGaGCUaGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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