Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 26567 | 5' | -54.4 | NC_005357.1 | + | 15378 | 0.66 | 0.716759 |
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Target: 5'- cGGUgagCGCGUccGCGUGCGGGccggCGa -3' miRNA: 3'- cCUAa--GCGCGu-CGCACGCCCaaa-GCa -5' |
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| 26567 | 5' | -54.4 | NC_005357.1 | + | 25792 | 0.66 | 0.705809 |
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Target: 5'- cGAUgCGCGCGGCGcGCucGGUaUCGUu -3' miRNA: 3'- cCUAaGCGCGUCGCaCGc-CCAaAGCA- -5' |
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| 26567 | 5' | -54.4 | NC_005357.1 | + | 1916 | 0.66 | 0.705809 |
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Target: 5'- aGGAcUUGCGCAGCagcuUGUGGGUg---- -3' miRNA: 3'- -CCUaAGCGCGUCGc---ACGCCCAaagca -5' |
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| 26567 | 5' | -54.4 | NC_005357.1 | + | 38923 | 0.66 | 0.683697 |
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Target: 5'- cGGGUucUCGCGCuGCGcucugguacaGCGGGccgUCGUu -3' miRNA: 3'- -CCUA--AGCGCGuCGCa---------CGCCCaa-AGCA- -5' |
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| 26567 | 5' | -54.4 | NC_005357.1 | + | 300 | 0.68 | 0.582938 |
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Target: 5'- cGGAaggCGCGCucuuccguccagGGCGUGCcguaccGGGggUCGUg -3' miRNA: 3'- -CCUaa-GCGCG------------UCGCACG------CCCaaAGCA- -5' |
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| 26567 | 5' | -54.4 | NC_005357.1 | + | 8131 | 0.68 | 0.571826 |
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Target: 5'- cGGcgUUG-GUugugGGCGUGCGGGUgUCGg -3' miRNA: 3'- -CCuaAGCgCG----UCGCACGCCCAaAGCa -5' |
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| 26567 | 5' | -54.4 | NC_005357.1 | + | 4398 | 0.69 | 0.53883 |
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Target: 5'- ---gUCGCGCcgggccuugAGCGUGCGGcagUCGUa -3' miRNA: 3'- ccuaAGCGCG---------UCGCACGCCcaaAGCA- -5' |
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| 26567 | 5' | -54.4 | NC_005357.1 | + | 10404 | 0.7 | 0.475044 |
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Target: 5'- cGGGUUCaaugucguggGCGUccAGCGUGUcgaGGGUUUCGc -3' miRNA: 3'- -CCUAAG----------CGCG--UCGCACG---CCCAAAGCa -5' |
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| 26567 | 5' | -54.4 | NC_005357.1 | + | 14764 | 0.71 | 0.415254 |
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Target: 5'- ----gCGCGCAGCGUGUcGGUgUCGa -3' miRNA: 3'- ccuaaGCGCGUCGCACGcCCAaAGCa -5' |
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| 26567 | 5' | -54.4 | NC_005357.1 | + | 21985 | 1.09 | 0.000881 |
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Target: 5'- cGGAUUCGCGCAGCGUGCGGGUUUCGUc -3' miRNA: 3'- -CCUAAGCGCGUCGCACGCCCAAAGCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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