Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 2657 | 3' | -55.1 | NC_001491.2 | + | 2979 | 0.69 | 0.82118 |
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Target: 5'- gGCCUucUGCGGcUGGGCUgCUGCCCcCGGg -3' miRNA: 3'- -CGGA--GCGUCuACUUGG-GAUGGGaGCC- -5' |
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| 2657 | 3' | -55.1 | NC_001491.2 | + | 1459 | 0.7 | 0.776567 |
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Target: 5'- aGCCUCGguGAUGGgcagcaccagggGCUCgg-CCUCGGc -3' miRNA: 3'- -CGGAGCguCUACU------------UGGGaugGGAGCC- -5' |
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| 2657 | 3' | -55.1 | NC_001491.2 | + | 2724 | 0.7 | 0.757764 |
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Target: 5'- aGCCUgGUggcccgGGGUGGccACCCUGCgCUUGGg -3' miRNA: 3'- -CGGAgCG------UCUACU--UGGGAUGgGAGCC- -5' |
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| 2657 | 3' | -55.1 | NC_001491.2 | + | 21521 | 0.71 | 0.718908 |
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Target: 5'- cGCUUCGCAGGUGGGCaa---CUUCGGg -3' miRNA: 3'- -CGGAGCGUCUACUUGggaugGGAGCC- -5' |
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| 2657 | 3' | -55.1 | NC_001491.2 | + | 35664 | 0.71 | 0.708987 |
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Target: 5'- gGCCggCGCGGcgcugGGGCCgUggaGCCCUCGGa -3' miRNA: 3'- -CGGa-GCGUCua---CUUGGgA---UGGGAGCC- -5' |
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| 2657 | 3' | -55.1 | NC_001491.2 | + | 55715 | 0.75 | 0.527433 |
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Target: 5'- aGCUgUCGCAGAaGAGCCucugCUACCCUCGu -3' miRNA: 3'- -CGG-AGCGUCUaCUUGG----GAUGGGAGCc -5' |
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| 2657 | 3' | -55.1 | NC_001491.2 | + | 59178 | 0.77 | 0.398624 |
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Target: 5'- cGCCUCGCGGugGUGAGCCCcggcgaUACUCUCa- -3' miRNA: 3'- -CGGAGCGUC--UACUUGGG------AUGGGAGcc -5' |
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| 2657 | 3' | -55.1 | NC_001491.2 | + | 91544 | 1.01 | 0.013763 |
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Target: 5'- gGCCUCGCAGGUaAACCCUACCCUCGGg -3' miRNA: 3'- -CGGAGCGUCUAcUUGGGAUGGGAGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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