Results 21 - 30 of 30 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 26570 | 3' | -53.5 | NC_005357.1 | + | 40045 | 0.67 | 0.654836 |
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Target: 5'- ----aGCGaccgCCAUGCCGUGCGCc -3' miRNA: 3'- gcuaaCGCcaaaGGUACGGCGCGUGu -5' |
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| 26570 | 3' | -53.5 | NC_005357.1 | + | 28267 | 0.67 | 0.666202 |
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Target: 5'- -uGUUGCGca--CCAUGaCCGCGCGCu -3' miRNA: 3'- gcUAACGCcaaaGGUAC-GGCGCGUGu -5' |
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| 26570 | 3' | -53.5 | NC_005357.1 | + | 23720 | 0.66 | 0.700048 |
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Target: 5'- aGGUcGCGGg--CCAgUGCCucggugGCGCGCAg -3' miRNA: 3'- gCUAaCGCCaaaGGU-ACGG------CGCGUGU- -5' |
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| 26570 | 3' | -53.5 | NC_005357.1 | + | 18339 | 0.66 | 0.722277 |
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Target: 5'- ----aGCGGggugcCCGUGCCcCGCGCGg -3' miRNA: 3'- gcuaaCGCCaaa--GGUACGGcGCGUGU- -5' |
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| 26570 | 3' | -53.5 | NC_005357.1 | + | 37075 | 0.66 | 0.725582 |
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Target: 5'- gCGGUgcagGCGGccacgcagcgcgaugCCAUGCUGCGcCACGu -3' miRNA: 3'- -GCUAa---CGCCaaa------------GGUACGGCGC-GUGU- -5' |
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| 26570 | 3' | -53.5 | NC_005357.1 | + | 41877 | 0.66 | 0.733255 |
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Target: 5'- uCGAggcGUGGuUUUUCAgcgGcCCGCGCACGu -3' miRNA: 3'- -GCUaa-CGCC-AAAGGUa--C-GGCGCGUGU- -5' |
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| 26570 | 3' | -53.5 | NC_005357.1 | + | 16175 | 0.66 | 0.733255 |
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Target: 5'- aGGccgGCGGgugcCCGUGCCuucggcgacGCGCGCAa -3' miRNA: 3'- gCUaa-CGCCaaa-GGUACGG---------CGCGUGU- -5' |
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| 26570 | 3' | -53.5 | NC_005357.1 | + | 21019 | 0.66 | 0.733255 |
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Target: 5'- uGAacGUGGaguucgUUUCCAUGCuCGCGCAg- -3' miRNA: 3'- gCUaaCGCC------AAAGGUACG-GCGCGUgu -5' |
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| 26570 | 3' | -53.5 | NC_005357.1 | + | 3742 | 0.66 | 0.744123 |
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Target: 5'- cCGGUgGUGGgcacgUCgGUGCCGcCGCugGa -3' miRNA: 3'- -GCUAaCGCCaa---AGgUACGGC-GCGugU- -5' |
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| 26570 | 3' | -53.5 | NC_005357.1 | + | 27460 | 0.66 | 0.744123 |
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Target: 5'- ----cGCuGGUaugCCAgcgcGCCGCGCACGc -3' miRNA: 3'- gcuaaCG-CCAaa-GGUa---CGGCGCGUGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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