Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 26576 | 5' | -58.6 | NC_005357.1 | + | 11362 | 0.66 | 0.527073 |
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Target: 5'- --cGGUGGCGAccugcucGCcCGgcaCCUCGGUGGc -3' miRNA: 3'- cccCUACCGCU-------CGuGCa--GGAGCCACC- -5' |
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| 26576 | 5' | -58.6 | NC_005357.1 | + | 28855 | 0.66 | 0.507465 |
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Target: 5'- --cGAUGGCGAGCGCGcguUCaacaUCGccGUGGg -3' miRNA: 3'- cccCUACCGCUCGUGC---AGg---AGC--CACC- -5' |
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| 26576 | 5' | -58.6 | NC_005357.1 | + | 39588 | 0.68 | 0.41023 |
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Target: 5'- cGGcuUGGCGGGCugGccuuUCCUCaauaGGUGGu -3' miRNA: 3'- cCCcuACCGCUCGugC----AGGAG----CCACC- -5' |
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| 26576 | 5' | -58.6 | NC_005357.1 | + | 24507 | 0.68 | 0.381636 |
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Target: 5'- aGGGGcUGGCGcccacgccgauCACGUCCa-GGUGGa -3' miRNA: 3'- -CCCCuACCGCuc---------GUGCAGGagCCACC- -5' |
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| 26576 | 5' | -58.6 | NC_005357.1 | + | 10562 | 0.69 | 0.354402 |
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Target: 5'- aGGGGGUcuugcacGGCGAGgGCGUgCCgcaggaauucuucgUCGGUGa -3' miRNA: 3'- -CCCCUA-------CCGCUCgUGCA-GG--------------AGCCACc -5' |
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| 26576 | 5' | -58.6 | NC_005357.1 | + | 30580 | 0.7 | 0.295363 |
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Target: 5'- cGGcaGAUGGUGGGCugGgauaCgUCGGUGGc -3' miRNA: 3'- cCC--CUACCGCUCGugCa---GgAGCCACC- -5' |
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| 26576 | 5' | -58.6 | NC_005357.1 | + | 8607 | 0.7 | 0.281155 |
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Target: 5'- uGGGGAUGGCGuaggcguggccGGUACGcCCUuCGGa-- -3' miRNA: 3'- -CCCCUACCGC-----------UCGUGCaGGA-GCCacc -5' |
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| 26576 | 5' | -58.6 | NC_005357.1 | + | 27958 | 1.13 | 0.000221 |
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Target: 5'- aGGGGAUGGCGAGCACGUCCUCGGUGGg -3' miRNA: 3'- -CCCCUACCGCUCGUGCAGGAGCCACC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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