Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 26578 | 5' | -51.5 | NC_005357.1 | + | 15602 | 0.66 | 0.858576 |
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Target: 5'- aCCGC-GCCAUCG-GC-CAgGAUGCuGa -3' miRNA: 3'- -GGCGaCGGUAGCuUGaGUaCUACGuC- -5' |
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| 26578 | 5' | -51.5 | NC_005357.1 | + | 10002 | 0.66 | 0.840734 |
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Target: 5'- gUCGCUGCCGUCGucuuGGCagaCGUGgcGCGc -3' miRNA: 3'- -GGCGACGGUAGC----UUGa--GUACuaCGUc -5' |
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| 26578 | 5' | -51.5 | NC_005357.1 | + | 36130 | 0.69 | 0.694191 |
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Target: 5'- -aGCUGCCGUCaAugUCGUGG-GCGa -3' miRNA: 3'- ggCGACGGUAGcUugAGUACUaCGUc -5' |
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| 26578 | 5' | -51.5 | NC_005357.1 | + | 34455 | 0.69 | 0.682807 |
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Target: 5'- gCUGCUGCCugaCGAACUCAUcguUGCc- -3' miRNA: 3'- -GGCGACGGua-GCUUGAGUAcu-ACGuc -5' |
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| 26578 | 5' | -51.5 | NC_005357.1 | + | 1124 | 0.69 | 0.671371 |
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Target: 5'- -gGCuUGCCGUCGA--UCAUGA-GCAGc -3' miRNA: 3'- ggCG-ACGGUAGCUugAGUACUaCGUC- -5' |
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| 26578 | 5' | -51.5 | NC_005357.1 | + | 17429 | 0.69 | 0.648389 |
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Target: 5'- cCCGCUGUCGUCGGcccccuuuguGCUgGUGGguggGCGc -3' miRNA: 3'- -GGCGACGGUAGCU----------UGAgUACUa---CGUc -5' |
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| 26578 | 5' | -51.5 | NC_005357.1 | + | 37328 | 0.7 | 0.636867 |
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Target: 5'- aCCGCUGCUAaccaagCGAACaUCA--AUGCAGc -3' miRNA: 3'- -GGCGACGGUa-----GCUUG-AGUacUACGUC- -5' |
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| 26578 | 5' | -51.5 | NC_005357.1 | + | 6331 | 0.7 | 0.613822 |
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Target: 5'- -aGC-GUCGUCGAACUCGcccUGGUGCGu -3' miRNA: 3'- ggCGaCGGUAGCUUGAGU---ACUACGUc -5' |
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| 26578 | 5' | -51.5 | NC_005357.1 | + | 7204 | 0.73 | 0.448252 |
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Target: 5'- -gGCuUGCCAUCGGACUgCGUGAUGaAGc -3' miRNA: 3'- ggCG-ACGGUAGCUUGA-GUACUACgUC- -5' |
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| 26578 | 5' | -51.5 | NC_005357.1 | + | 28942 | 1.11 | 0.001362 |
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Target: 5'- cCCGCUGCCAUCGAACUCAUGAUGCAGc -3' miRNA: 3'- -GGCGACGGUAGCUUGAGUACUACGUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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