miRNA: 3'- auaGUGGCACA--AACC------AUCUGCgCuCG- -5'
miRNA: 3'- auagugGCACAAACCa---UCUGCGCu-CG- -5'
miRNA: 3'- aUAGUGg-C-ACaa--ACCauCUGCGCUCG- -5'
miRNA: 3'- -AUAGUGGCACAAACCAUCUGCGCUCG- -5'
Results 1 - 4 of 4 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 2658 | 3' | -50.5 | NC_001491.2 | + | 124700 | 0.66 | 0.991576 |
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Target: 5'- --cCGCCGUGUuuUUGGccaacgUAGACGgGcGCa -3' miRNA: 3'- auaGUGGCACA--AACC------AUCUGCgCuCG- -5' |
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| 2658 | 3' | -50.5 | NC_001491.2 | + | 43770 | 0.67 | 0.990345 |
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Target: 5'- -----aCGUGUUUGGgcgaGGACGCGcuGCg -3' miRNA: 3'- auagugGCACAAACCa---UCUGCGCu-CG- -5' |
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| 2658 | 3' | -50.5 | NC_001491.2 | + | 118073 | 0.68 | 0.974778 |
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Target: 5'- gAUCACgaGcUGaaacUGGgcGACGCGAGCg -3' miRNA: 3'- aUAGUGg-C-ACaa--ACCauCUGCGCUCG- -5' |
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| 2658 | 3' | -50.5 | NC_001491.2 | + | 89919 | 1.1 | 0.007869 |
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Target: 5'- aUAUCACCGUGUUUGGUAGACGCGAGCc -3' miRNA: 3'- -AUAGUGGCACAAACCAUCUGCGCUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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