Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 26580 | 3' | -59.5 | NC_005357.1 | + | 25481 | 0.68 | 0.28926 |
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Target: 5'- cGAUGAgGGCGCGCucgugcuuggucagGGUCAGgcCCGCGa -3' miRNA: 3'- -CUGCU-UCGCGCG--------------CCGGUCaaGGCGCa -5' |
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| 26580 | 3' | -59.5 | NC_005357.1 | + | 3003 | 0.69 | 0.284245 |
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Target: 5'- gGACGAAG-GCGCGGCgGGUcauagCUGCu- -3' miRNA: 3'- -CUGCUUCgCGCGCCGgUCAa----GGCGca -5' |
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| 26580 | 3' | -59.5 | NC_005357.1 | + | 14587 | 0.69 | 0.2772 |
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Target: 5'- -uUGAgcAGCgGCGCGGCCGGcgccaCCGCGg -3' miRNA: 3'- cuGCU--UCG-CGCGCCGGUCaa---GGCGCa -5' |
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| 26580 | 3' | -59.5 | NC_005357.1 | + | 24005 | 0.69 | 0.2772 |
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Target: 5'- uGGCGAaggugGGCGCGuCGGCCAaUUCaGCGa -3' miRNA: 3'- -CUGCU-----UCGCGC-GCCGGUcAAGgCGCa -5' |
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| 26580 | 3' | -59.5 | NC_005357.1 | + | 14727 | 0.69 | 0.256903 |
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Target: 5'- cGGCGAgccgcugccGGCGCGCGGCCuGUauUCCaaGa -3' miRNA: 3'- -CUGCU---------UCGCGCGCCGGuCA--AGGcgCa -5' |
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| 26580 | 3' | -59.5 | NC_005357.1 | + | 21569 | 0.71 | 0.187669 |
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Target: 5'- cACGgcGCGCGCcgucgugcagucGGUCAGUUcCCGCGa -3' miRNA: 3'- cUGCuuCGCGCG------------CCGGUCAA-GGCGCa -5' |
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| 26580 | 3' | -59.5 | NC_005357.1 | + | 16088 | 0.75 | 0.096829 |
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Target: 5'- uACG-AGCgGCGCGGCCAGUU-CGCGg -3' miRNA: 3'- cUGCuUCG-CGCGCCGGUCAAgGCGCa -5' |
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| 26580 | 3' | -59.5 | NC_005357.1 | + | 28620 | 0.76 | 0.086458 |
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Target: 5'- aACcGGGCGCGgaugaggcCGGCCAGUUCCGCGc -3' miRNA: 3'- cUGcUUCGCGC--------GCCGGUCAAGGCGCa -5' |
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| 26580 | 3' | -59.5 | NC_005357.1 | + | 29472 | 1.06 | 0.000417 |
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Target: 5'- cGACGAAGCGCGCGGCCAGUUCCGCGUc -3' miRNA: 3'- -CUGCUUCGCGCGCCGGUCAAGGCGCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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