Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 26581 | 5' | -60 | NC_005357.1 | + | 17834 | 0.66 | 0.437503 |
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Target: 5'- uCGGGCGGGUUgauGC-CCGCCaCCguggCCUCg -3' miRNA: 3'- -GCUUGCCCAGau-CGuGGUGG-GG----GGGG- -5' |
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| 26581 | 5' | -60 | NC_005357.1 | + | 34797 | 0.67 | 0.391689 |
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Target: 5'- gGcgUGGGUCga--GCCGCgCCCCCUg -3' miRNA: 3'- gCuuGCCCAGaucgUGGUGgGGGGGG- -5' |
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| 26581 | 5' | -60 | NC_005357.1 | + | 9989 | 0.67 | 0.372545 |
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Target: 5'- uGAGCguggcgGGGUCgaaagugccgcGCGCCcgGCCCCCgCCCu -3' miRNA: 3'- gCUUG------CCCAGau---------CGUGG--UGGGGG-GGG- -5' |
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| 26581 | 5' | -60 | NC_005357.1 | + | 33414 | 0.67 | 0.357362 |
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Target: 5'- aCGAACuGGU---GCGCCGCCUgcuggaagcgCCCCCg -3' miRNA: 3'- -GCUUGcCCAgauCGUGGUGGG----------GGGGG- -5' |
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| 26581 | 5' | -60 | NC_005357.1 | + | 35475 | 0.73 | 0.139106 |
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Target: 5'- cCGAGCGGGUCaAGCGUCAauaCCCCCa -3' miRNA: 3'- -GCUUGCCCAGaUCGUGGUgggGGGGG- -5' |
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| 26581 | 5' | -60 | NC_005357.1 | + | 17226 | 0.74 | 0.128202 |
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Target: 5'- aGGACGcGGacgUUGGaCAUCACCCCCUCCa -3' miRNA: 3'- gCUUGC-CCa--GAUC-GUGGUGGGGGGGG- -5' |
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| 26581 | 5' | -60 | NC_005357.1 | + | 29874 | 1.12 | 0.000179 |
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Target: 5'- gCGAACGGGUCUAGCACCACCCCCCCCg -3' miRNA: 3'- -GCUUGCCCAGAUCGUGGUGGGGGGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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