miRNA display CGI


Results 1 - 17 of 17 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
26582 3' -53.5 NC_005357.1 + 26768 0.66 0.749444
Target:  5'- gUACUCgGGCGAgccgguaagCCAUAucagcggccuGGAGCACCu -3'
miRNA:   3'- -GUGAG-CCGCUa--------GGUGUu---------CCUUGUGGu -5'
26582 3' -53.5 NC_005357.1 + 40544 0.66 0.727756
Target:  5'- aGCg-GGCGAagCGCAAGGu-CGCCAa -3'
miRNA:   3'- gUGagCCGCUagGUGUUCCuuGUGGU- -5'
26582 3' -53.5 NC_005357.1 + 13885 0.66 0.705661
Target:  5'- gGC-CGGCGG-CCGCAAGGucgUGCCGu -3'
miRNA:   3'- gUGaGCCGCUaGGUGUUCCuu-GUGGU- -5'
26582 3' -53.5 NC_005357.1 + 13353 0.66 0.705661
Target:  5'- -cCUCGGCG--CC-CGAGGAugACCc -3'
miRNA:   3'- guGAGCCGCuaGGuGUUCCUugUGGu -5'
26582 3' -53.5 NC_005357.1 + 24178 0.67 0.683258
Target:  5'- gCGCuUCGGCGuGUCCAUGAGcgcGAGCACg- -3'
miRNA:   3'- -GUG-AGCCGC-UAGGUGUUC---CUUGUGgu -5'
26582 3' -53.5 NC_005357.1 + 24074 0.67 0.671973
Target:  5'- gCACaguagCGGCGA-CCGCGcGGGGCACgGg -3'
miRNA:   3'- -GUGa----GCCGCUaGGUGUuCCUUGUGgU- -5'
26582 3' -53.5 NC_005357.1 + 4613 0.67 0.671973
Target:  5'- -cCUCGGCGAugaugUCgGCGGGGAugcuGgGCCAc -3'
miRNA:   3'- guGAGCCGCU-----AGgUGUUCCU----UgUGGU- -5'
26582 3' -53.5 NC_005357.1 + 40367 0.67 0.671973
Target:  5'- uGCUgGGCGAcgaCCcCGAGGAAUugCGc -3'
miRNA:   3'- gUGAgCCGCUa--GGuGUUCCUUGugGU- -5'
26582 3' -53.5 NC_005357.1 + 18173 0.67 0.649293
Target:  5'- aCGCUCaaGGUGGcaagCCGCGAGG-ACAUCAu -3'
miRNA:   3'- -GUGAG--CCGCUa---GGUGUUCCuUGUGGU- -5'
26582 3' -53.5 NC_005357.1 + 7539 0.68 0.626546
Target:  5'- gCGCUCGGCGGcgugcucgaUCCGCG----GCGCCAc -3'
miRNA:   3'- -GUGAGCCGCU---------AGGUGUuccuUGUGGU- -5'
26582 3' -53.5 NC_005357.1 + 33518 0.69 0.569947
Target:  5'- cCGCU-GGCGAgaaaggagaacUCCGCAuccagugguGGGACGCCAa -3'
miRNA:   3'- -GUGAgCCGCU-----------AGGUGUu--------CCUUGUGGU- -5'
26582 3' -53.5 NC_005357.1 + 7929 0.69 0.558753
Target:  5'- gCGCUCGGCG-UCCACcuuc-ACGCCAc -3'
miRNA:   3'- -GUGAGCCGCuAGGUGuuccuUGUGGU- -5'
26582 3' -53.5 NC_005357.1 + 22254 0.71 0.421494
Target:  5'- gACaaGGCGAagCCGCAAGGcGCACCc -3'
miRNA:   3'- gUGagCCGCUa-GGUGUUCCuUGUGGu -5'
26582 3' -53.5 NC_005357.1 + 18337 0.71 0.411772
Target:  5'- aGCUUGGUGGg-CAUAGGGAugACCAc -3'
miRNA:   3'- gUGAGCCGCUagGUGUUCCUugUGGU- -5'
26582 3' -53.5 NC_005357.1 + 28718 0.72 0.392749
Target:  5'- gCugUCGGCGca-CGCGAGGAugaGCGCCu -3'
miRNA:   3'- -GugAGCCGCuagGUGUUCCU---UGUGGu -5'
26582 3' -53.5 NC_005357.1 + 4582 0.76 0.211374
Target:  5'- uGCUCGGUGcgCCagGCGAGGAACugCu -3'
miRNA:   3'- gUGAGCCGCuaGG--UGUUCCUUGugGu -5'
26582 3' -53.5 NC_005357.1 + 30229 1.09 0.001127
Target:  5'- gCACUCGGCGAUCCACAAGGAACACCAa -3'
miRNA:   3'- -GUGAGCCGCUAGGUGUUCCUUGUGGU- -5' 
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.