miRNA: 3'- uuauaCGCGACuGCGuaAGUCUACcc -5'
miRNA: 3'- uuaUACGCGACUGCG------UAAGUCUaccc -5'
miRNA: 3'- uuauaCGCGACUGCGuaa----GUCuaCCC- -5'
miRNA: 3'- uuaUACGCGACUGCGUAAGuCUACCc -5'
miRNA: 3'- -UUAUACGCGACUGCGUAAgUcUACCC- -5'
Results 1 - 5 of 5 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 26585 | 3' | -49.6 | NC_005357.1 | + | 473 | 0.67 | 0.864561 |
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Target: 5'- ----gGCGCUGuCGCggUCGGAUGc- -3' miRNA: 3'- uuauaCGCGACuGCGuaAGUCUACcc -5' |
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| 26585 | 3' | -49.6 | NC_005357.1 | + | 35877 | 0.68 | 0.817774 |
|
Target: 5'- --gAUGCGCUGACGCcuggcgAUUCGGGc--- -3' miRNA: 3'- uuaUACGCGACUGCG------UAAGUCUaccc -5' |
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| 26585 | 3' | -49.6 | NC_005357.1 | + | 7179 | 0.7 | 0.685822 |
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Target: 5'- ----cGCGCUGACGCccugacgCAGccGGGa -3' miRNA: 3'- uuauaCGCGACUGCGuaa----GUCuaCCC- -5' |
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| 26585 | 3' | -49.6 | NC_005357.1 | + | 11706 | 0.84 | 0.122104 |
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Target: 5'- --aAUGCGUUGACGCAUUCcGAUGGa -3' miRNA: 3'- uuaUACGCGACUGCGUAAGuCUACCc -5' |
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| 26585 | 3' | -49.6 | NC_005357.1 | + | 31749 | 0.87 | 0.072643 |
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Target: 5'- aAAUAUGCGCUGACGCAUUgAcAUGGGc -3' miRNA: 3'- -UUAUACGCGACUGCGUAAgUcUACCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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