Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 26585 | 5' | -49.4 | NC_005357.1 | + | 24545 | 0.66 | 0.874149 |
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Target: 5'- -aCCGCGUGGUcGCGCa-GCGCGg--- -3' miRNA: 3'- aaGGCGUAUUA-CGCGgaUGCGUaaau -5' |
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| 26585 | 5' | -49.4 | NC_005357.1 | + | 35942 | 0.66 | 0.865459 |
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Target: 5'- --gCGCGUGaAUGCggGCCUGCGCGg--- -3' miRNA: 3'- aagGCGUAU-UACG--CGGAUGCGUaaau -5' |
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| 26585 | 5' | -49.4 | NC_005357.1 | + | 27281 | 0.67 | 0.835744 |
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Target: 5'- -aCCGCAguucgccgcGCGCCUugGCGa--- -3' miRNA: 3'- aaGGCGUauua-----CGCGGAugCGUaaau -5' |
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| 26585 | 5' | -49.4 | NC_005357.1 | + | 19715 | 0.67 | 0.817851 |
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Target: 5'- -gCUGCAUAGagGCGCC-GCGCAUc-- -3' miRNA: 3'- aaGGCGUAUUa-CGCGGaUGCGUAaau -5' |
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| 26585 | 5' | -49.4 | NC_005357.1 | + | 24729 | 0.69 | 0.741817 |
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Target: 5'- -gCCGCuuccaccGCGCCUGCGCGg--- -3' miRNA: 3'- aaGGCGuauua--CGCGGAUGCGUaaau -5' |
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| 26585 | 5' | -49.4 | NC_005357.1 | + | 1670 | 0.69 | 0.706943 |
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Target: 5'- uUUgCGCGUGAUGCuGCC-GCGCAggUAg -3' miRNA: 3'- -AAgGCGUAUUACG-CGGaUGCGUaaAU- -5' |
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| 26585 | 5' | -49.4 | NC_005357.1 | + | 11502 | 0.69 | 0.706943 |
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Target: 5'- -aCCGCAaAGggcaGCGCCUGCGCu---- -3' miRNA: 3'- aaGGCGUaUUa---CGCGGAUGCGuaaau -5' |
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| 26585 | 5' | -49.4 | NC_005357.1 | + | 25611 | 0.72 | 0.539771 |
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Target: 5'- -gCCGCGUGAacUGCGCCgccuggGCGCGa--- -3' miRNA: 3'- aaGGCGUAUU--ACGCGGa-----UGCGUaaau -5' |
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| 26585 | 5' | -49.4 | NC_005357.1 | + | 31784 | 1.05 | 0.003723 |
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Target: 5'- uUUCCGCAUAAUGCGCCUACGCAUUUAa -3' miRNA: 3'- -AAGGCGUAUUACGCGGAUGCGUAAAU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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