miRNA: 3'- -CUugGGcCGCGCGAGCG--UGUUGa---UAC- -5'
miRNA: 3'- cUUGGGCCG-CGCG---AGC-GUGUUGauac -5'
miRNA: 3'- cuuGGGCCGCgcGAGCGUGUUGauac -5'
miRNA: 3'- cUUGGGCCG--CGCGaGCG-----UGUUGa--UAC- -5'
miRNA: 3'- cUUGG-GCCGC-GCGAGCGuGUUGauac -5'
Results 41 - 45 of 45 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 26586 | 3' | -55.4 | NC_005357.1 | + | 12392 | 0.66 | 0.614105 |
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Target: 5'- cGAugCCuGCGCGCUUGUauGCGGCcuugAUGc -3' miRNA: 3'- -CUugGGcCGCGCGAGCG--UGUUGa---UAC- -5' |
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| 26586 | 3' | -55.4 | NC_005357.1 | + | 9315 | 0.66 | 0.636672 |
|
Target: 5'- cGACCUGGCgGCGCacgUCGgCGCGGCg--- -3' miRNA: 3'- cUUGGGCCG-CGCG---AGC-GUGUUGauac -5' |
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| 26586 | 3' | -55.4 | NC_005357.1 | + | 12160 | 0.66 | 0.636672 |
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Target: 5'- --uUCCGGCGa-CUCGCGCGACc--- -3' miRNA: 3'- cuuGGGCCGCgcGAGCGUGUUGauac -5' |
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| 26586 | 3' | -55.4 | NC_005357.1 | + | 41746 | 0.66 | 0.636672 |
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Target: 5'- cGGCCCGGCcgGCGC-CGCcguagGCGGCcugGUGg -3' miRNA: 3'- cUUGGGCCG--CGCGaGCG-----UGUUGa--UAC- -5' |
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| 26586 | 3' | -55.4 | NC_005357.1 | + | 7361 | 0.66 | 0.636672 |
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Target: 5'- cGACCuCGGCGuCGUUCGUcCAGCg--- -3' miRNA: 3'- cUUGG-GCCGC-GCGAGCGuGUUGauac -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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