Results 41 - 45 of 45 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
26586 | 3' | -55.4 | NC_005357.1 | + | 38041 | 0.68 | 0.461887 |
Target: 5'- aGGCCCGGCGCGacggCGCcUGGCUGc- -3' miRNA: 3'- cUUGGGCCGCGCga--GCGuGUUGAUac -5' |
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26586 | 3' | -55.4 | NC_005357.1 | + | 38270 | 0.66 | 0.614105 |
Target: 5'- cGACCaCGGCGCGCaaggUGgGCGGCUu-- -3' miRNA: 3'- cUUGG-GCCGCGCGa---GCgUGUUGAuac -5' |
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26586 | 3' | -55.4 | NC_005357.1 | + | 38389 | 0.74 | 0.218737 |
Target: 5'- -cGCCCGGCuGCGCUCgacccGCGCGGC-AUGa -3' miRNA: 3'- cuUGGGCCG-CGCGAG-----CGUGUUGaUAC- -5' |
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26586 | 3' | -55.4 | NC_005357.1 | + | 38854 | 0.74 | 0.230846 |
Target: 5'- uGACCCGGCGCG-UCGCugAAauUUAUGc -3' miRNA: 3'- cUUGGGCCGCGCgAGCGugUU--GAUAC- -5' |
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26586 | 3' | -55.4 | NC_005357.1 | + | 41746 | 0.66 | 0.636672 |
Target: 5'- cGGCCCGGCcgGCGC-CGCcguagGCGGCcugGUGg -3' miRNA: 3'- cUUGGGCCG--CGCGaGCG-----UGUUGa--UAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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