Results 21 - 30 of 30 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 26591 | 3' | -58.7 | NC_005357.1 | + | 22589 | 0.71 | 0.186549 |
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Target: 5'- cACGCUGCUGaacaaaACCGgCCUGGGCa-- -3' miRNA: 3'- uUGCGAUGGCg-----UGGCaGGACCCGaug -5' |
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| 26591 | 3' | -58.7 | NC_005357.1 | + | 37320 | 0.72 | 0.181529 |
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Target: 5'- cGGCGCcaGCCGCACCGgacgCUGGGCcgGCc -3' miRNA: 3'- -UUGCGa-UGGCGUGGCag--GACCCGa-UG- -5' |
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| 26591 | 3' | -58.7 | NC_005357.1 | + | 35222 | 0.72 | 0.171848 |
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Target: 5'- aGAUGCUGCCGUggacaucgcGCUGUCCc-GGCUGCg -3' miRNA: 3'- -UUGCGAUGGCG---------UGGCAGGacCCGAUG- -5' |
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| 26591 | 3' | -58.7 | NC_005357.1 | + | 25613 | 0.72 | 0.16263 |
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Target: 5'- cGCGUgaACUGCGCCG-CCUGGGCgcgACc -3' miRNA: 3'- uUGCGa-UGGCGUGGCaGGACCCGa--UG- -5' |
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| 26591 | 3' | -58.7 | NC_005357.1 | + | 16809 | 0.72 | 0.15819 |
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Target: 5'- cGCGCUgcuggcgACCGCGCCGccgugguugccacugCCUGGGCcGCa -3' miRNA: 3'- uUGCGA-------UGGCGUGGCa--------------GGACCCGaUG- -5' |
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| 26591 | 3' | -58.7 | NC_005357.1 | + | 14429 | 0.72 | 0.15819 |
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Target: 5'- cGCGcCUGCgGCACCGUgUUGGGCg-- -3' miRNA: 3'- uUGC-GAUGgCGUGGCAgGACCCGaug -5' |
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| 26591 | 3' | -58.7 | NC_005357.1 | + | 13044 | 0.73 | 0.153859 |
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Target: 5'- uGGC-CUACCGCGCCGagUCCgaGGGCUGg -3' miRNA: 3'- -UUGcGAUGGCGUGGC--AGGa-CCCGAUg -5' |
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| 26591 | 3' | -58.7 | NC_005357.1 | + | 27690 | 0.74 | 0.119489 |
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Target: 5'- cAAUGCUGCgCGUACUG-CCUgGGGCUACc -3' miRNA: 3'- -UUGCGAUG-GCGUGGCaGGA-CCCGAUG- -5' |
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| 26591 | 3' | -58.7 | NC_005357.1 | + | 35841 | 0.74 | 0.116144 |
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Target: 5'- uACGUggUGCCG-GCCGUgCUGGGCUACu -3' miRNA: 3'- uUGCG--AUGGCgUGGCAgGACCCGAUG- -5' |
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| 26591 | 3' | -58.7 | NC_005357.1 | + | 33568 | 1.08 | 0.000339 |
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Target: 5'- gAACGCUACCGCACCGUCCUGGGCUACg -3' miRNA: 3'- -UUGCGAUGGCGUGGCAGGACCCGAUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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