Results 61 - 63 of 63 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
2660 | 5' | -59.2 | NC_001491.2 | + | 24699 | 0.77 | 0.241732 |
Target: 5'- --cGCCCGCUCGguccacgGC-CGCCGCCGCGa -3' miRNA: 3'- ccaUGGGCGAGC-------UGaGUGGCGGCGCg -5' |
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2660 | 5' | -59.2 | NC_001491.2 | + | 25056 | 0.78 | 0.220588 |
Target: 5'- -cUGCgCGCUCG-CUCGCCGCCGCa- -3' miRNA: 3'- ccAUGgGCGAGCuGAGUGGCGGCGcg -5' |
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2660 | 5' | -59.2 | NC_001491.2 | + | 89204 | 1.13 | 0.000888 |
Target: 5'- uGGUACCCGCUCGACUCACCGCCGCGCa -3' miRNA: 3'- -CCAUGGGCGAGCUGAGUGGCGGCGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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