miRNA: 3'- ccaUGG-GCGagCUGAGUGgCGGCGCG- -5'
miRNA: 3'- ccAUG-GGCGA-----------GCuGAGugGCGGCGCG- -5'
miRNA: 3'- ccaUGGGCGa-GcUGAGugGcGGCGCG- -5'
Results 61 - 63 of 63 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 2660 | 5' | -59.2 | NC_001491.2 | + | 136910 | 0.74 | 0.347079 |
|
Target: 5'- --aGCCaCGC--GACUCACgGCCGCGCa -3' miRNA: 3'- ccaUGG-GCGagCUGAGUGgCGGCGCG- -5' |
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| 2660 | 5' | -59.2 | NC_001491.2 | + | 143587 | 0.66 | 0.786928 |
|
Target: 5'- -uUGCuCUGCUgcugugcgaagCGuCUCugCGCCGCGUg -3' miRNA: 3'- ccAUG-GGCGA-----------GCuGAGugGCGGCGCG- -5' |
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| 2660 | 5' | -59.2 | NC_001491.2 | + | 148444 | 0.66 | 0.812836 |
|
Target: 5'- --cGCCCGCggCcGCUCugCcCCGCGUu -3' miRNA: 3'- ccaUGGGCGa-GcUGAGugGcGGCGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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