Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 26600 | 5' | -55.5 | NC_005357.1 | + | 16799 | 0.7 | 0.37976 |
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Target: 5'- cCGAGGUGgacGCgCUGC----UGGCGACCg -3' miRNA: 3'- -GUUCCACa--CG-GACGugcuACCGCUGG- -5' |
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| 26600 | 5' | -55.5 | NC_005357.1 | + | 11348 | 0.73 | 0.267738 |
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Target: 5'- -cAGGUcuUGCa-GUACGGUGGCGACCu -3' miRNA: 3'- guUCCAc-ACGgaCGUGCUACCGCUGG- -5' |
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| 26600 | 5' | -55.5 | NC_005357.1 | + | 33756 | 0.74 | 0.214294 |
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Target: 5'- cCGAGGUGcgcgcggcacgcaaUGCCgaccGCGCGAUgaaggccGGCGACCu -3' miRNA: 3'- -GUUCCAC--------------ACGGa---CGUGCUA-------CCGCUGG- -5' |
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| 26600 | 5' | -55.5 | NC_005357.1 | + | 18376 | 0.76 | 0.151433 |
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Target: 5'- gCGAGGUuggccuUGCCgaugGCGcCGGUGGCGGCCa -3' miRNA: 3'- -GUUCCAc-----ACGGa---CGU-GCUACCGCUGG- -5' |
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| 26600 | 5' | -55.5 | NC_005357.1 | + | 26603 | 0.77 | 0.143145 |
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Target: 5'- -cGGcGUGUucgaGuCCUGCGCGGUGGUGGCCg -3' miRNA: 3'- guUC-CACA----C-GGACGUGCUACCGCUGG- -5' |
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| 26600 | 5' | -55.5 | NC_005357.1 | + | 35738 | 1.12 | 0.000415 |
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Target: 5'- aCAAGGUGUGCCUGCACGAUGGCGACCu -3' miRNA: 3'- -GUUCCACACGGACGUGCUACCGCUGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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