miRNA: 3'- -GCGGuaCGGGCUgauaGGCgaCCGCG- -5'
miRNA: 3'- -GCGGuaCGGGC-----UGAUa---GGCG-----------ACCGCG- -5'
miRNA: 3'- -GCGGUACGGGCUGAUAGGCGACCGCG- -5'
Results 61 - 63 of 63 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 26604 | 3' | -58.8 | NC_005357.1 | + | 6352 | 0.74 | 0.145328 |
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Target: 5'- gCGCCcgGCCCGAagg-CCGg-GGCGCu -3' miRNA: 3'- -GCGGuaCGGGCUgauaGGCgaCCGCG- -5' |
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| 26604 | 3' | -58.8 | NC_005357.1 | + | 12724 | 0.75 | 0.119708 |
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Target: 5'- uCGCCcgGCCCGuugguGCUGggugCCGCgcccagacgcuUGGCGCg -3' miRNA: 3'- -GCGGuaCGGGC-----UGAUa---GGCG-----------ACCGCG- -5' |
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| 26604 | 3' | -58.8 | NC_005357.1 | + | 36618 | 1.11 | 0.000212 |
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Target: 5'- aCGCCAUGCCCGACUAUCCGCUGGCGCu -3' miRNA: 3'- -GCGGUACGGGCUGAUAGGCGACCGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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