Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 26607 | 5' | -58.3 | NC_005357.1 | + | 29692 | 0.67 | 0.35495 |
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Target: 5'- cGUCUGGgcGCGGcacCCAGCACCaacggGCCGg -3' miRNA: 3'- aUAGGCCaaCGUCc--GGUCGUGG-----UGGU- -5' |
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| 26607 | 5' | -58.3 | NC_005357.1 | + | 30146 | 0.67 | 0.35495 |
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Target: 5'- --gCCGGcacGCuGGGCCAGCAacCCGCCc -3' miRNA: 3'- auaGGCCaa-CG-UCCGGUCGU--GGUGGu -5' |
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| 26607 | 5' | -58.3 | NC_005357.1 | + | 37220 | 1.07 | 0.000395 |
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Target: 5'- gUAUCCGGUUGCAGGCCAGCACCACCAg -3' miRNA: 3'- -AUAGGCCAACGUCCGGUCGUGGUGGU- -5' |
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| 26607 | 5' | -58.3 | NC_005357.1 | + | 40673 | 0.68 | 0.298248 |
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Target: 5'- --gCCaGgcGCAGGCCGGCcaugACCGCCc -3' miRNA: 3'- auaGGcCaaCGUCCGGUCG----UGGUGGu -5' |
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| 26607 | 5' | -58.3 | NC_005357.1 | + | 41146 | 0.66 | 0.399875 |
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Target: 5'- ---gCGG-UGUGGGCgGGCAUCACCu -3' miRNA: 3'- auagGCCaACGUCCGgUCGUGGUGGu -5' |
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| 26607 | 5' | -58.3 | NC_005357.1 | + | 41736 | 0.73 | 0.156671 |
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Target: 5'- gUAUCuUGGgcgGCccGGCCGGCGCCGCCGu -3' miRNA: 3'- -AUAG-GCCaa-CGu-CCGGUCGUGGUGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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