Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 2661 | 3' | -56.2 | NC_001491.2 | + | 74022 | 0.66 | 0.914282 |
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Target: 5'- uCUGcUCUCUGUGCCuaga-CCCCAUGc -3' miRNA: 3'- -GGC-AGAGGCGCGGuuaacGGGGUACa -5' |
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| 2661 | 3' | -56.2 | NC_001491.2 | + | 42156 | 0.66 | 0.908324 |
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Target: 5'- gUCGUCUCCccgGCGCCAggUGagacaggagcucCCCCAc-- -3' miRNA: 3'- -GGCAGAGG---CGCGGUuaAC------------GGGGUaca -5' |
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| 2661 | 3' | -56.2 | NC_001491.2 | + | 148436 | 0.66 | 0.882181 |
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Target: 5'- gCCGUCcacgcCCGCgGCCGcucUGCCCCGcGUu -3' miRNA: 3'- -GGCAGa----GGCG-CGGUua-ACGGGGUaCA- -5' |
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| 2661 | 3' | -56.2 | NC_001491.2 | + | 84572 | 0.67 | 0.875084 |
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Target: 5'- aCCGUgaCCGCGCa----GCCCCAa-- -3' miRNA: 3'- -GGCAgaGGCGCGguuaaCGGGGUaca -5' |
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| 2661 | 3' | -56.2 | NC_001491.2 | + | 26963 | 0.67 | 0.865536 |
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Target: 5'- aCCGgcgCCGCGCCGAgauccacgagguaccGCCCCAc-- -3' miRNA: 3'- -GGCagaGGCGCGGUUaa-------------CGGGGUaca -5' |
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| 2661 | 3' | -56.2 | NC_001491.2 | + | 31809 | 0.67 | 0.860248 |
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Target: 5'- gCCGUCUCCGacucCCGA--GCCCCGc-- -3' miRNA: 3'- -GGCAGAGGCgc--GGUUaaCGGGGUaca -5' |
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| 2661 | 3' | -56.2 | NC_001491.2 | + | 5896 | 0.68 | 0.818835 |
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Target: 5'- cCCGUCagauauccaggcuUCCGCGCCGA--GCUCCGc-- -3' miRNA: 3'- -GGCAG-------------AGGCGCGGUUaaCGGGGUaca -5' |
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| 2661 | 3' | -56.2 | NC_001491.2 | + | 143607 | 0.71 | 0.64651 |
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Target: 5'- gCGUCUCUGCGCCGcgUGgCUCUAa-- -3' miRNA: 3'- gGCAGAGGCGCGGUuaAC-GGGGUaca -5' |
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| 2661 | 3' | -56.2 | NC_001491.2 | + | 16197 | 0.74 | 0.506043 |
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Target: 5'- gCCGUCUCCGUucggccGCCGAUgcGCCCCccUGUc -3' miRNA: 3'- -GGCAGAGGCG------CGGUUAa-CGGGGu-ACA- -5' |
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| 2661 | 3' | -56.2 | NC_001491.2 | + | 88646 | 1.1 | 0.002393 |
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Target: 5'- aCCGUCUCCGCGCCAAUUGCCCCAUGUg -3' miRNA: 3'- -GGCAGAGGCGCGGUUAACGGGGUACA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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