Results 21 - 24 of 24 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
2661 | 5' | -52.3 | NC_001491.2 | + | 74860 | 0.73 | 0.773935 |
Target: 5'- uGGCAGuauACGGCAACgAGGGGAa--- -3' miRNA: 3'- gCUGUCu--UGCCGUUGgUCUCCUguac -5' |
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2661 | 5' | -52.3 | NC_001491.2 | + | 5345 | 0.75 | 0.631083 |
Target: 5'- cCGGCuGGGCGGCAGCCgccgggguaGGAGGACc-- -3' miRNA: 3'- -GCUGuCUUGCCGUUGG---------UCUCCUGuac -5' |
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2661 | 5' | -52.3 | NC_001491.2 | + | 139643 | 0.76 | 0.568401 |
Target: 5'- gGGCAGAagcgcauugACGGCAGCCAGAgcGGACu-- -3' miRNA: 3'- gCUGUCU---------UGCCGUUGGUCU--CCUGuac -5' |
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2661 | 5' | -52.3 | NC_001491.2 | + | 88680 | 1.1 | 0.005842 |
Target: 5'- cCGACAGAACGGCAACCAGAGGACAUGa -3' miRNA: 3'- -GCUGUCUUGCCGUUGGUCUCCUGUAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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