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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 26614 | 5' | -54.4 | NC_005357.1 | + | 8597 | 0.66 | 0.645712 |
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Target: 5'- -aCGGCGACcgUGGGGaUGGcguaGGCGUGg -3' miRNA: 3'- uaGCUGCUGaaGCUCC-ACCa---CCGUAC- -5' |
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| 26614 | 5' | -54.4 | NC_005357.1 | + | 26007 | 0.68 | 0.533409 |
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Target: 5'- uAUUGGCGAUUUCGAGGgcuuccucGGcgaUGGCAUc -3' miRNA: 3'- -UAGCUGCUGAAGCUCCa-------CC---ACCGUAc -5' |
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| 26614 | 5' | -54.4 | NC_005357.1 | + | 26602 | 0.74 | 0.267807 |
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Target: 5'- -aCGGCGugUUCGAguccugcgcGGUGGUGGCc-- -3' miRNA: 3'- uaGCUGCugAAGCU---------CCACCACCGuac -5' |
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| 26614 | 5' | -54.4 | NC_005357.1 | + | 26675 | 0.66 | 0.679611 |
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Target: 5'- cAUUGGCGGCaaCGAGGUGc-GGUAUGu -3' miRNA: 3'- -UAGCUGCUGaaGCUCCACcaCCGUAC- -5' |
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| 26614 | 5' | -54.4 | NC_005357.1 | + | 34853 | 0.69 | 0.486026 |
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Target: 5'- -cCGACGACUUCGuGGacguguccaagccGGUGGCGc- -3' miRNA: 3'- uaGCUGCUGAAGCuCCa------------CCACCGUac -5' |
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| 26614 | 5' | -54.4 | NC_005357.1 | + | 35986 | 0.66 | 0.679611 |
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Target: 5'- -gCGACGcCUUCGcGGGcGGUGGUGUc -3' miRNA: 3'- uaGCUGCuGAAGC-UCCaCCACCGUAc -5' |
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| 26614 | 5' | -54.4 | NC_005357.1 | + | 36354 | 0.77 | 0.154842 |
|
Target: 5'- cUCGACGACUUCGAGGcGGUGuG-AUGa -3' miRNA: 3'- uAGCUGCUGAAGCUCCaCCAC-CgUAC- -5' |
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| 26614 | 5' | -54.4 | NC_005357.1 | + | 37249 | 0.7 | 0.409347 |
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Target: 5'- uAUCGACGACgugC-AGGcGGUGGCAg- -3' miRNA: 3'- -UAGCUGCUGaa-GcUCCaCCACCGUac -5' |
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| 26614 | 5' | -54.4 | NC_005357.1 | + | 38771 | 0.66 | 0.69083 |
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Target: 5'- -gCGugGA---CGAGGUGcUGGCGUGa -3' miRNA: 3'- uaGCugCUgaaGCUCCACcACCGUAC- -5' |
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| 26614 | 5' | -54.4 | NC_005357.1 | + | 38980 | 1.07 | 0.00121 |
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Target: 5'- uAUCGACGACUUCGAGGUGGUGGCAUGa -3' miRNA: 3'- -UAGCUGCUGAAGCUCCACCACCGUAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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