Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 26619 | 3' | -54.4 | NC_005357.1 | + | 35695 | 0.69 | 0.540771 |
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Target: 5'- -cUAUUGAGCGcggCGGCCCaGCGCa -3' miRNA: 3'- uaGUAGCUUGCauaGCCGGGcUGCGc -5' |
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| 26619 | 3' | -54.4 | NC_005357.1 | + | 38677 | 0.67 | 0.640876 |
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Target: 5'- -aCGUCcAGCGg--CGGCaCCGACGUGc -3' miRNA: 3'- uaGUAGcUUGCauaGCCG-GGCUGCGC- -5' |
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| 26619 | 3' | -54.4 | NC_005357.1 | + | 39469 | 0.7 | 0.445428 |
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Target: 5'- uGUCAUCGAucACGUGcUCGacgugccGCgCGACGCGg -3' miRNA: 3'- -UAGUAGCU--UGCAU-AGC-------CGgGCUGCGC- -5' |
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| 26619 | 3' | -54.4 | NC_005357.1 | + | 40181 | 0.66 | 0.712028 |
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Target: 5'- -gCGUCGAACugccggccaaGGCCCGGCGUu -3' miRNA: 3'- uaGUAGCUUGcauag-----CCGGGCUGCGc -5' |
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| 26619 | 3' | -54.4 | NC_005357.1 | + | 40647 | 1.06 | 0.001541 |
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Target: 5'- aAUCAUCGAACGUAUCGGCCCGACGCGc -3' miRNA: 3'- -UAGUAGCUUGCAUAGCCGGGCUGCGC- -5' |
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| 26619 | 3' | -54.4 | NC_005357.1 | + | 41872 | 0.68 | 0.562713 |
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Target: 5'- cAUgGUCGAgGCGUGguuuuucagCGGCCCG-CGCa -3' miRNA: 3'- -UAgUAGCU-UGCAUa--------GCCGGGCuGCGc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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