Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 2662 | 5' | -58.3 | NC_001491.2 | + | 77367 | 0.67 | 0.764525 |
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Target: 5'- gUCUuUGUGGGCgGGCGcGCCUggcUCGGGUu -3' miRNA: 3'- gAGAuACGCUCG-CCGU-CGGG---AGUCCG- -5' |
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| 2662 | 5' | -58.3 | NC_001491.2 | + | 12185 | 0.67 | 0.764525 |
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Target: 5'- -----aGCGAGCGcGCAGCggaagaCCUCGGuGCc -3' miRNA: 3'- gagauaCGCUCGC-CGUCG------GGAGUC-CG- -5' |
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| 2662 | 5' | -58.3 | NC_001491.2 | + | 3725 | 0.67 | 0.764525 |
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Target: 5'- -----cGCGGccGCGGCAGCCUugcgUCGGGg -3' miRNA: 3'- gagauaCGCU--CGCCGUCGGG----AGUCCg -5' |
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| 2662 | 5' | -58.3 | NC_001491.2 | + | 39337 | 0.66 | 0.791291 |
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Target: 5'- aUCUGgcaguucUGCGGGUGaGCGGCCCaugcGGCc -3' miRNA: 3'- gAGAU-------ACGCUCGC-CGUCGGGagu-CCG- -5' |
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| 2662 | 5' | -58.3 | NC_001491.2 | + | 119208 | 0.66 | 0.801144 |
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Target: 5'- -----cGCGAGCuGUGcGCCCUgGGGCu -3' miRNA: 3'- gagauaCGCUCGcCGU-CGGGAgUCCG- -5' |
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| 2662 | 5' | -58.3 | NC_001491.2 | + | 33838 | 0.66 | 0.809943 |
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Target: 5'- cCUCgcgGUGCGcgccguucuGGUGGUGGCCCggaccguGGCg -3' miRNA: 3'- -GAGa--UACGC---------UCGCCGUCGGGagu----CCG- -5' |
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| 2662 | 5' | -58.3 | NC_001491.2 | + | 43977 | 0.66 | 0.818582 |
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Target: 5'- -----gGCGGGUGGCgacggacagcuGGCCCUCucggagAGGCc -3' miRNA: 3'- gagauaCGCUCGCCG-----------UCGGGAG------UCCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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