Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 2662 | 5' | -58.3 | NC_001491.2 | + | 22885 | 0.68 | 0.655989 |
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Target: 5'- aUCUucaguaguUGCGAGUGGCAGCuuuucacauacuCCUCugGGGUa -3' miRNA: 3'- gAGAu-------ACGCUCGCCGUCG------------GGAG--UCCG- -5' |
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| 2662 | 5' | -58.3 | NC_001491.2 | + | 12474 | 0.7 | 0.564873 |
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Target: 5'- uCUCccgcccgGCGGGCGGCGggcGCCCgCGGGUc -3' miRNA: 3'- -GAGaua----CGCUCGCCGU---CGGGaGUCCG- -5' |
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| 2662 | 5' | -58.3 | NC_001491.2 | + | 12185 | 0.67 | 0.764525 |
|
Target: 5'- -----aGCGAGCGcGCAGCggaagaCCUCGGuGCc -3' miRNA: 3'- gagauaCGCUCGC-CGUCG------GGAGUC-CG- -5' |
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| 2662 | 5' | -58.3 | NC_001491.2 | + | 4851 | 0.68 | 0.666122 |
|
Target: 5'- -----cGCcgGAGCGGCAGCuCUUCGGGg -3' miRNA: 3'- gagauaCG--CUCGCCGUCG-GGAGUCCg -5' |
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| 2662 | 5' | -58.3 | NC_001491.2 | + | 3953 | 0.71 | 0.515545 |
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Target: 5'- gCUCUucugaGUGCGAucguaGCGGCGGCUCauggccacCAGGCg -3' miRNA: 3'- -GAGA-----UACGCU-----CGCCGUCGGGa-------GUCCG- -5' |
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| 2662 | 5' | -58.3 | NC_001491.2 | + | 3827 | 0.68 | 0.666122 |
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Target: 5'- -gCUGgccGCGgcAGCGGCGGCCUgggcCGGGUa -3' miRNA: 3'- gaGAUa--CGC--UCGCCGUCGGGa---GUCCG- -5' |
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| 2662 | 5' | -58.3 | NC_001491.2 | + | 3725 | 0.67 | 0.764525 |
|
Target: 5'- -----cGCGGccGCGGCAGCCUugcgUCGGGg -3' miRNA: 3'- gagauaCGCU--CGCCGUCGGG----AGUCCg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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