Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 26623 | 5' | -57.5 | NC_005357.1 | + | 7624 | 0.66 | 0.501498 |
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Target: 5'- gUGAUGGUcaGGGGgcGcggcucgacccACGCCGGCu--- -3' miRNA: 3'- -ACUACCG--CCCCuaC-----------UGCGGCCGuaag -5' |
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| 26623 | 5' | -57.5 | NC_005357.1 | + | 8164 | 0.66 | 0.512035 |
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Target: 5'- cGAUGauGCGGcccaGGAaGGCGCUGGCGUc- -3' miRNA: 3'- aCUAC--CGCC----CCUaCUGCGGCCGUAag -5' |
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| 26623 | 5' | -57.5 | NC_005357.1 | + | 36887 | 0.66 | 0.513094 |
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Target: 5'- -cGUGGCGGGcGAccugggcggaaccugGGCgGCCGGCGUg- -3' miRNA: 3'- acUACCGCCC-CUa--------------CUG-CGGCCGUAag -5' |
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| 26623 | 5' | -57.5 | NC_005357.1 | + | 30584 | 0.66 | 0.522663 |
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Target: 5'- aGAUGGUGGGcugggauacgucGGUGGCaUCGGCGaUCu -3' miRNA: 3'- aCUACCGCCC------------CUACUGcGGCCGUaAG- -5' |
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| 26623 | 5' | -57.5 | NC_005357.1 | + | 21089 | 0.66 | 0.522663 |
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Target: 5'- cGuUGGCaaccuGGGcGcgGugGCCGGCAUc- -3' miRNA: 3'- aCuACCG-----CCC-CuaCugCGGCCGUAag -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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