Results 61 - 69 of 69 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 26631 | 3' | -68.1 | NC_005808.1 | + | 5583 | 0.66 | 0.161611 |
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Target: 5'- uGGGCGG-CCGGCguguccuacgacuuUGGCaGCauCGCCGUAc -3' miRNA: 3'- -CCCGCCgGGCCG--------------GCCG-CG--GCGGCAUc -5' |
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| 26631 | 3' | -68.1 | NC_005808.1 | + | 17009 | 0.66 | 0.16285 |
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Target: 5'- cGGCGGCaCGcuGCUGGaCGgCGCCGUGc -3' miRNA: 3'- cCCGCCGgGC--CGGCC-GCgGCGGCAUc -5' |
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| 26631 | 3' | -68.1 | NC_005808.1 | + | 3645 | 0.66 | 0.16285 |
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Target: 5'- uGGCGGCCgCGGaUCGGUGUaCGCCc--- -3' miRNA: 3'- cCCGCCGG-GCC-GGCCGCG-GCGGcauc -5' |
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| 26631 | 3' | -68.1 | NC_005808.1 | + | 2204 | 0.66 | 0.16704 |
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Target: 5'- cGGCGGCgCGGuugaCCGGCuGCUGCaCGa-- -3' miRNA: 3'- cCCGCCGgGCC----GGCCG-CGGCG-GCauc -5' |
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| 26631 | 3' | -68.1 | NC_005808.1 | + | 22490 | 0.66 | 0.16704 |
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Target: 5'- gGGGCgGGCUgGGCUGGUcauGCCcGCCa--- -3' miRNA: 3'- -CCCG-CCGGgCCGGCCG---CGG-CGGcauc -5' |
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| 26631 | 3' | -68.1 | NC_005808.1 | + | 29088 | 0.66 | 0.16704 |
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Target: 5'- cGGGCgccgaggucaGGCCCGuGCgGGacuuGCUGCCGg-- -3' miRNA: 3'- -CCCG----------CCGGGC-CGgCCg---CGGCGGCauc -5' |
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| 26631 | 3' | -68.1 | NC_005808.1 | + | 27692 | 0.66 | 0.171327 |
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Target: 5'- uGGaauacaGGCCgCGcGCCGGCagcggcucGCCGCCGg-- -3' miRNA: 3'- cCCg-----CCGG-GC-CGGCCG--------CGGCGGCauc -5' |
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| 26631 | 3' | -68.1 | NC_005808.1 | + | 9210 | 0.66 | 0.175712 |
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Target: 5'- cGGCGGCgCGGCgcacguucugCGGCGCgC-CCGgcGg -3' miRNA: 3'- cCCGCCGgGCCG----------GCCGCG-GcGGCauC- -5' |
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| 26631 | 3' | -68.1 | NC_005808.1 | + | 14396 | 0.66 | 0.175712 |
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Target: 5'- -aGCaGGCCCGGCaguucagccaaGGCGCgCGCCa--- -3' miRNA: 3'- ccCG-CCGGGCCGg----------CCGCG-GCGGcauc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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