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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 26636 | 5' | -56.6 | NC_005808.1 | + | 28656 | 0.68 | 0.418764 |
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Target: 5'- aGUgCCagAUCGGUgccggcGGCCUCGCCagCGCGg -3' miRNA: 3'- -UAgGGa-UAGCCA------UCGGAGUGGa-GCGU- -5' |
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| 26636 | 5' | -56.6 | NC_005808.1 | + | 23208 | 0.66 | 0.554145 |
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Target: 5'- -gCCUcGUCGGUgaauucGGCCUCgGCCUCGg- -3' miRNA: 3'- uaGGGaUAGCCA------UCGGAG-UGGAGCgu -5' |
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| 26636 | 5' | -56.6 | NC_005808.1 | + | 18032 | 0.68 | 0.42458 |
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Target: 5'- gGUUCUUGUCGGcgggcugcuugggGGCCUUACCggCGCGg -3' miRNA: 3'- -UAGGGAUAGCCa------------UCGGAGUGGa-GCGU- -5' |
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| 26636 | 5' | -56.6 | NC_005808.1 | + | 10097 | 0.66 | 0.543156 |
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Target: 5'- cAUCCUcgacaaaaagGUCGGUAguGCCUguCCUCGUAc -3' miRNA: 3'- -UAGGGa---------UAGCCAU--CGGAguGGAGCGU- -5' |
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| 26636 | 5' | -56.6 | NC_005808.1 | + | 8966 | 0.66 | 0.554145 |
|
Target: 5'- cAUCCgcacGUCGGUAacGCCUCGauuuCCUCGCc -3' miRNA: 3'- -UAGGga--UAGCCAU--CGGAGU----GGAGCGu -5' |
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| 26636 | 5' | -56.6 | NC_005808.1 | + | 2249 | 1.07 | 0.000666 |
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Target: 5'- cAUCCCUAUCGGUAGCCUCACCUCGCAa -3' miRNA: 3'- -UAGGGAUAGCCAUCGGAGUGGAGCGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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