Results 1 - 8 of 8 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
26638 | 5' | -49.4 | NC_005808.1 | + | 39645 | 0.67 | 0.865955 |
Target: 5'- -cGAUCacgUGCUCGA--CGUGCCGCGc- -3' miRNA: 3'- uuUUAG---ACGAGCUacGCAUGGCGUuc -5' |
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26638 | 5' | -49.4 | NC_005808.1 | + | 9700 | 0.67 | 0.857124 |
Target: 5'- ----gCUGCUCaacGUGUACCGCGAc -3' miRNA: 3'- uuuuaGACGAGcuaCGCAUGGCGUUc -5' |
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26638 | 5' | -49.4 | NC_005808.1 | + | 39916 | 0.7 | 0.73315 |
Target: 5'- cGGcgCUGCUCGG-GCGUGCCGa--- -3' miRNA: 3'- uUUuaGACGAGCUaCGCAUGGCguuc -5' |
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26638 | 5' | -49.4 | NC_005808.1 | + | 38089 | 0.7 | 0.686858 |
Target: 5'- gAGGcgCUGCUCGGUGC--GCCagGCGAGg -3' miRNA: 3'- -UUUuaGACGAGCUACGcaUGG--CGUUC- -5' |
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26638 | 5' | -49.4 | NC_005808.1 | + | 5692 | 0.71 | 0.66329 |
Target: 5'- cAAAAUCUcGCUCGAUGacagACCGCcAGa -3' miRNA: 3'- -UUUUAGA-CGAGCUACgca-UGGCGuUC- -5' |
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26638 | 5' | -49.4 | NC_005808.1 | + | 28063 | 0.71 | 0.627715 |
Target: 5'- -cGGUCUGCUCGAacaGCGggcaggugGCCGCGGc -3' miRNA: 3'- uuUUAGACGAGCUa--CGCa-------UGGCGUUc -5' |
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26638 | 5' | -49.4 | NC_005808.1 | + | 31933 | 0.76 | 0.356615 |
Target: 5'- gGGGGUCuUGCacggCGAggGCGUGCCGCAGGa -3' miRNA: 3'- -UUUUAG-ACGa---GCUa-CGCAUGGCGUUC- -5' |
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26638 | 5' | -49.4 | NC_005808.1 | + | 2737 | 1.08 | 0.002962 |
Target: 5'- gAAAAUCUGCUCGAUGCGUACCGCAAGa -3' miRNA: 3'- -UUUUAGACGAGCUACGCAUGGCGUUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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