Results 1 - 15 of 15 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
26639 | 3' | -48.8 | NC_005808.1 | + | 4143 | 1.15 | 0.001449 |
Target: 5'- cGCAGAUCAACGAAUACAGCCUGUGGGa -3' miRNA: 3'- -CGUCUAGUUGCUUAUGUCGGACACCC- -5' |
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26639 | 3' | -48.8 | NC_005808.1 | + | 4882 | 0.69 | 0.819677 |
Target: 5'- cCAGcgCGGCGAcgGCGGCCUcguuuucggccacgGUGGa -3' miRNA: 3'- cGUCuaGUUGCUuaUGUCGGA--------------CACCc -5' |
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26639 | 3' | -48.8 | NC_005808.1 | + | 8098 | 0.69 | 0.83224 |
Target: 5'- gGCGG--CAAgGAcgGCGGCCcuaUGUGGGa -3' miRNA: 3'- -CGUCuaGUUgCUuaUGUCGG---ACACCC- -5' |
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26639 | 3' | -48.8 | NC_005808.1 | + | 10948 | 0.66 | 0.953276 |
Target: 5'- gGCAGuugcGCGGcgACAGCCg--GGGa -3' miRNA: 3'- -CGUCuaguUGCUuaUGUCGGacaCCC- -5' |
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26639 | 3' | -48.8 | NC_005808.1 | + | 12258 | 0.67 | 0.932657 |
Target: 5'- aCGGAUCGGCG-GUAguGuCCUGggcGGGu -3' miRNA: 3'- cGUCUAGUUGCuUAUguC-GGACa--CCC- -5' |
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26639 | 3' | -48.8 | NC_005808.1 | + | 14648 | 0.67 | 0.930324 |
Target: 5'- aGCGGAUCGGCcgccggcauCAGCCcgGUaGGGg -3' miRNA: 3'- -CGUCUAGUUGcuuau----GUCGGa-CA-CCC- -5' |
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26639 | 3' | -48.8 | NC_005808.1 | + | 20059 | 0.66 | 0.955071 |
Target: 5'- aGCAaGUUGGCGAGUucuacgcggacaucgGCGGCCUGcccgaaaccUGGGu -3' miRNA: 3'- -CGUcUAGUUGCUUA---------------UGUCGGAC---------ACCC- -5' |
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26639 | 3' | -48.8 | NC_005808.1 | + | 20857 | 0.66 | 0.948093 |
Target: 5'- uGCAGAUCAcCGAcauggacGUGCAGCCg----- -3' miRNA: 3'- -CGUCUAGUuGCU-------UAUGUCGGacaccc -5' |
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26639 | 3' | -48.8 | NC_005808.1 | + | 24310 | 0.7 | 0.781955 |
Target: 5'- -gAGGUCGGCGA--GCAGCCgc-GGGu -3' miRNA: 3'- cgUCUAGUUGCUuaUGUCGGacaCCC- -5' |
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26639 | 3' | -48.8 | NC_005808.1 | + | 27390 | 0.67 | 0.913941 |
Target: 5'- cGUGGAaagCAACGc--GC-GCCUGUGGGc -3' miRNA: 3'- -CGUCUa--GUUGCuuaUGuCGGACACCC- -5' |
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26639 | 3' | -48.8 | NC_005808.1 | + | 29979 | 0.67 | 0.920491 |
Target: 5'- gGCAcuGAUgcGCGAacugGUACGGCCUGUcaugccGGGg -3' miRNA: 3'- -CGU--CUAguUGCU----UAUGUCGGACA------CCC- -5' |
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26639 | 3' | -48.8 | NC_005808.1 | + | 31106 | 0.66 | 0.94358 |
Target: 5'- uGCAGGcCGGCGAGUGguGCgaGauagcgGGGa -3' miRNA: 3'- -CGUCUaGUUGCUUAUguCGgaCa-----CCC- -5' |
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26639 | 3' | -48.8 | NC_005808.1 | + | 33936 | 0.69 | 0.822611 |
Target: 5'- aGCAGccgcugcacaCAGCGAGUacggcGCGGCCUG-GGGc -3' miRNA: 3'- -CGUCua--------GUUGCUUA-----UGUCGGACaCCC- -5' |
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26639 | 3' | -48.8 | NC_005808.1 | + | 36443 | 0.68 | 0.899916 |
Target: 5'- cGCuGGgaaGAUGAcgGCGGCgaGUGGGu -3' miRNA: 3'- -CGuCUag-UUGCUuaUGUCGgaCACCC- -5' |
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26639 | 3' | -48.8 | NC_005808.1 | + | 40727 | 0.71 | 0.726173 |
Target: 5'- cGCGGAUCGucuGCGggUucuggucgagcgcGCGGCCcUG-GGGg -3' miRNA: 3'- -CGUCUAGU---UGCuuA-------------UGUCGG-ACaCCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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