Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 2664 | 5' | -54.7 | NC_001491.2 | + | 33829 | 0.66 | 0.909646 |
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Target: 5'- -cGAGCUGCGCCUC-GCGGUg----- -3' miRNA: 3'- auCUCGACGUGGGGuCGUCAaacucc -5' |
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| 2664 | 5' | -54.7 | NC_001491.2 | + | 47856 | 0.67 | 0.896745 |
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Target: 5'- gUAGAGCggaaaaaCACCCC-GCGcUUUGAGGc -3' miRNA: 3'- -AUCUCGac-----GUGGGGuCGUcAAACUCC- -5' |
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| 2664 | 5' | -54.7 | NC_001491.2 | + | 148921 | 0.67 | 0.875584 |
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Target: 5'- -uGGGCUGgGCCCCAcGCuGgcugcgGGGGg -3' miRNA: 3'- auCUCGACgUGGGGU-CGuCaaa---CUCC- -5' |
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| 2664 | 5' | -54.7 | NC_001491.2 | + | 95736 | 0.68 | 0.852363 |
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Target: 5'- aUAGAGUaucUGC-CUCCAGCAGccgccuggGAGGg -3' miRNA: 3'- -AUCUCG---ACGuGGGGUCGUCaaa-----CUCC- -5' |
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| 2664 | 5' | -54.7 | NC_001491.2 | + | 25134 | 0.68 | 0.835821 |
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Target: 5'- gGGGGCcGCcaACUCCAGCGGcgagGAGGc -3' miRNA: 3'- aUCUCGaCG--UGGGGUCGUCaaa-CUCC- -5' |
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| 2664 | 5' | -54.7 | NC_001491.2 | + | 4266 | 0.68 | 0.835821 |
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Target: 5'- cUGGGGcCUGCGCCUCGGCGuagcgcGAGGc -3' miRNA: 3'- -AUCUC-GACGUGGGGUCGUcaaa--CUCC- -5' |
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| 2664 | 5' | -54.7 | NC_001491.2 | + | 11485 | 0.69 | 0.772228 |
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Target: 5'- cGGGGCUGCcugGCCCCucuGGggUGGGGg -3' miRNA: 3'- aUCUCGACG---UGGGGucgUCaaACUCC- -5' |
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| 2664 | 5' | -54.7 | NC_001491.2 | + | 133235 | 0.72 | 0.650116 |
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Target: 5'- aGGGGCUGCaagaguguaACCCCGGagAGUgcuaUGAGGg -3' miRNA: 3'- aUCUCGACG---------UGGGGUCg-UCAa---ACUCC- -5' |
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| 2664 | 5' | -54.7 | NC_001491.2 | + | 84822 | 0.95 | 0.025311 |
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Target: 5'- cUAGAGCUG-ACCCCAGCAGUUUGAGGc -3' miRNA: 3'- -AUCUCGACgUGGGGUCGUCAAACUCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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