Results 21 - 35 of 35 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
26640 | 5' | -54.9 | NC_005808.1 | + | 12294 | 0.68 | 0.488344 |
Target: 5'- -gCCGCUguugccgGUGUGCUgcgucccgaccagGGGcAGGCAGCGCg -3' miRNA: 3'- aaGGUGA-------UACGCGG-------------CCC-UUCGUUGCG- -5' |
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26640 | 5' | -54.9 | NC_005808.1 | + | 23592 | 0.68 | 0.479887 |
Target: 5'- -aCCGC-GUGCGCCGccuGGAAGgcGCGCu -3' miRNA: 3'- aaGGUGaUACGCGGC---CCUUCguUGCG- -5' |
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26640 | 5' | -54.9 | NC_005808.1 | + | 12156 | 0.69 | 0.459065 |
Target: 5'- cUUCCGCguagGCGUCGGGGucAGUguccGAUGCg -3' miRNA: 3'- -AAGGUGaua-CGCGGCCCU--UCG----UUGCG- -5' |
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26640 | 5' | -54.9 | NC_005808.1 | + | 11437 | 0.69 | 0.448836 |
Target: 5'- -gCCACcgagGUGCCGGGcGAGCAGguCGCc -3' miRNA: 3'- aaGGUGaua-CGCGGCCC-UUCGUU--GCG- -5' |
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26640 | 5' | -54.9 | NC_005808.1 | + | 9947 | 0.69 | 0.428764 |
Target: 5'- -gCCACgc-GCcugGCCGaGAAGCAGCGCg -3' miRNA: 3'- aaGGUGauaCG---CGGCcCUUCGUUGCG- -5' |
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26640 | 5' | -54.9 | NC_005808.1 | + | 32442 | 0.69 | 0.418929 |
Target: 5'- cUUCCuGCUggGCgGCCGGGGccucGGCGGCGa -3' miRNA: 3'- -AAGG-UGAuaCG-CGGCCCU----UCGUUGCg -5' |
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26640 | 5' | -54.9 | NC_005808.1 | + | 31017 | 0.7 | 0.390263 |
Target: 5'- gUCCACgg-GCuuGCCGGccGAGGCAaucGCGCg -3' miRNA: 3'- aAGGUGauaCG--CGGCC--CUUCGU---UGCG- -5' |
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26640 | 5' | -54.9 | NC_005808.1 | + | 27958 | 0.7 | 0.380997 |
Target: 5'- aUCCGCgacGUGCUGGGcGAGCA-CGCc -3' miRNA: 3'- aAGGUGauaCGCGGCCC-UUCGUuGCG- -5' |
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26640 | 5' | -54.9 | NC_005808.1 | + | 31530 | 0.7 | 0.371878 |
Target: 5'- -cUCGCUGUGuUGCCGuGGAAaCAGCGCc -3' miRNA: 3'- aaGGUGAUAC-GCGGC-CCUUcGUUGCG- -5' |
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26640 | 5' | -54.9 | NC_005808.1 | + | 6729 | 0.7 | 0.371878 |
Target: 5'- -gCCGCU--GCGCUGGGccGCcGCGCu -3' miRNA: 3'- aaGGUGAuaCGCGGCCCuuCGuUGCG- -5' |
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26640 | 5' | -54.9 | NC_005808.1 | + | 17700 | 0.71 | 0.36291 |
Target: 5'- -gCCACUcgG-GCCGGGAaaugcccaucgcGGCAagcuGCGCg -3' miRNA: 3'- aaGGUGAuaCgCGGCCCU------------UCGU----UGCG- -5' |
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26640 | 5' | -54.9 | NC_005808.1 | + | 42379 | 0.73 | 0.274368 |
Target: 5'- gUCCAgUAgcUGCGCCGGaAGGC-GCGCu -3' miRNA: 3'- aAGGUgAU--ACGCGGCCcUUCGuUGCG- -5' |
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26640 | 5' | -54.9 | NC_005808.1 | + | 25141 | 0.73 | 0.246747 |
Target: 5'- --gCGCgcUGCGCUGGGuGGCGAUGCu -3' miRNA: 3'- aagGUGauACGCGGCCCuUCGUUGCG- -5' |
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26640 | 5' | -54.9 | NC_005808.1 | + | 38877 | 0.77 | 0.129038 |
Target: 5'- aUCCGCgagcaugagcggAUGCGCCGcauGggGCAGCGCg -3' miRNA: 3'- aAGGUGa-----------UACGCGGCc--CuuCGUUGCG- -5' |
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26640 | 5' | -54.9 | NC_005808.1 | + | 4199 | 1.09 | 0.000656 |
Target: 5'- cUUCCACUAUGCGCCGGGAAGCAACGCu -3' miRNA: 3'- -AAGGUGAUACGCGGCCCUUCGUUGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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