Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 26649 | 3' | -52.7 | NC_005808.1 | + | 8133 | 0.67 | 0.746751 |
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Target: 5'- gCGUUg--GUUGUGGGCGugCgGgugUCGg -3' miRNA: 3'- -GCAGuuaCAGCACCCGCugGaCa--AGC- -5' |
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| 26649 | 3' | -52.7 | NC_005808.1 | + | 30603 | 0.7 | 0.579631 |
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Target: 5'- aCGUCGGUGgcaucGGCGAUCUGUUCu -3' miRNA: 3'- -GCAGUUACagcacCCGCUGGACAAGc -5' |
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| 26649 | 3' | -52.7 | NC_005808.1 | + | 5346 | 0.71 | 0.492332 |
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Target: 5'- uGUCGAUGUCGUuGGCGACagccaacaUGcUCGa -3' miRNA: 3'- gCAGUUACAGCAcCCGCUGg-------ACaAGC- -5' |
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| 26649 | 3' | -52.7 | NC_005808.1 | + | 32088 | 0.75 | 0.315012 |
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Target: 5'- -uUCAAUGUCGUGGGCGuCCaGcgugUCGa -3' miRNA: 3'- gcAGUUACAGCACCCGCuGGaCa---AGC- -5' |
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| 26649 | 3' | -52.7 | NC_005808.1 | + | 5618 | 0.76 | 0.263023 |
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Target: 5'- cCGUCGAUaccGUgGcGGGCGACCUGggCGg -3' miRNA: 3'- -GCAGUUA---CAgCaCCCGCUGGACaaGC- -5' |
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| 26649 | 3' | -52.7 | NC_005808.1 | + | 6359 | 1.11 | 0.001216 |
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Target: 5'- cCGUCAAUGUCGUGGGCGACCUGUUCGg -3' miRNA: 3'- -GCAGUUACAGCACCCGCUGGACAAGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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