Results 1 - 20 of 23 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
26650 | 3' | -59.9 | NC_005808.1 | + | 874 | 0.68 | 0.293377 |
Target: 5'- cGGCaaggGCu---GGGCG-CGGCGCGUCGc -3' miRNA: 3'- -CCGa---CGcaguCCCGCaGUCGCGCAGC- -5' |
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26650 | 3' | -59.9 | NC_005808.1 | + | 1631 | 0.66 | 0.435779 |
Target: 5'- gGGC-GUGUCguacccaucGGGGUagGUCuGCGCGUCc -3' miRNA: 3'- -CCGaCGCAG---------UCCCG--CAGuCGCGCAGc -5' |
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26650 | 3' | -59.9 | NC_005808.1 | + | 4859 | 0.67 | 0.3639 |
Target: 5'- aGGUUGCccgGUCAGGuCGUugccCAGCGCGgCGa -3' miRNA: 3'- -CCGACG---CAGUCCcGCA----GUCGCGCaGC- -5' |
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26650 | 3' | -59.9 | NC_005808.1 | + | 7244 | 1.11 | 0.000188 |
Target: 5'- cGGCUGCGUCAGGGCGUCAGCGCGUCGg -3' miRNA: 3'- -CCGACGCAGUCCCGCAGUCGCGCAGC- -5' |
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26650 | 3' | -59.9 | NC_005808.1 | + | 8127 | 0.67 | 0.35468 |
Target: 5'- cGGCcgGCGUUGguugugggcgugcGGGUGUCGGCgcuGCGUCc -3' miRNA: 3'- -CCGa-CGCAGU-------------CCCGCAGUCG---CGCAGc -5' |
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26650 | 3' | -59.9 | NC_005808.1 | + | 8553 | 0.66 | 0.417041 |
Target: 5'- aGGCcGCGcUCGaccucauccuGGGCGUCGuaggccGCGCGUgCGg -3' miRNA: 3'- -CCGaCGC-AGU----------CCCGCAGU------CGCGCA-GC- -5' |
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26650 | 3' | -59.9 | NC_005808.1 | + | 8826 | 0.72 | 0.166323 |
Target: 5'- uGCUGgGUCGGGccagccgcaacGCGggcCAGCGCGUUGg -3' miRNA: 3'- cCGACgCAGUCC-----------CGCa--GUCGCGCAGC- -5' |
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26650 | 3' | -59.9 | NC_005808.1 | + | 12203 | 0.66 | 0.435779 |
Target: 5'- cGGaaGCG-CAGcGCGU-GGCGCGUCGc -3' miRNA: 3'- -CCgaCGCaGUCcCGCAgUCGCGCAGC- -5' |
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26650 | 3' | -59.9 | NC_005808.1 | + | 12278 | 0.72 | 0.161485 |
Target: 5'- uGCUGCGUCccgaccaGGGGCaggCAGCGCGggcCGg -3' miRNA: 3'- cCGACGCAG-------UCCCGca-GUCGCGCa--GC- -5' |
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26650 | 3' | -59.9 | NC_005808.1 | + | 12494 | 0.69 | 0.265631 |
Target: 5'- aGCcGCGcaCAGuGGCGUuuuucaugaaCAGCGCGUCGa -3' miRNA: 3'- cCGaCGCa-GUC-CCGCA----------GUCGCGCAGC- -5' |
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26650 | 3' | -59.9 | NC_005808.1 | + | 14401 | 0.66 | 0.417041 |
Target: 5'- aGGCguaGCGaagCuGGGCGUCgccaGGCGCGccugCGg -3' miRNA: 3'- -CCGa--CGCa--GuCCCGCAG----UCGCGCa---GC- -5' |
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26650 | 3' | -59.9 | NC_005808.1 | + | 14744 | 0.67 | 0.3639 |
Target: 5'- aGGCaguacGCG-CAGcauuGGCGcgCAGCGUGUCGg -3' miRNA: 3'- -CCGa----CGCaGUC----CCGCa-GUCGCGCAGC- -5' |
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26650 | 3' | -59.9 | NC_005808.1 | + | 16142 | 0.69 | 0.258382 |
Target: 5'- aGGaCUGCGgccgCAcgcugauggugguGGGCGUUAcCGCGUCGg -3' miRNA: 3'- -CC-GACGCa---GU-------------CCCGCAGUcGCGCAGC- -5' |
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26650 | 3' | -59.9 | NC_005808.1 | + | 18045 | 0.66 | 0.426349 |
Target: 5'- gGGCUGCuUgGGGGCcuuacCGGCGCGgggugCGu -3' miRNA: 3'- -CCGACGcAgUCCCGca---GUCGCGCa----GC- -5' |
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26650 | 3' | -59.9 | NC_005808.1 | + | 20615 | 0.66 | 0.407859 |
Target: 5'- aGGCgGCGUCGcaggucGGUGUCGGCcuGCGcUCGu -3' miRNA: 3'- -CCGaCGCAGUc-----CCGCAGUCG--CGC-AGC- -5' |
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26650 | 3' | -59.9 | NC_005808.1 | + | 24598 | 0.66 | 0.398804 |
Target: 5'- cGGC-GUGUCcuugccGGGGUagGUCAGCGgcaCGUCGa -3' miRNA: 3'- -CCGaCGCAG------UCCCG--CAGUCGC---GCAGC- -5' |
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26650 | 3' | -59.9 | NC_005808.1 | + | 25063 | 0.68 | 0.314873 |
Target: 5'- cGCUGuCGUCGGcccccuuugugcuGGUggGUgGGCGCGUCGa -3' miRNA: 3'- cCGAC-GCAGUC-------------CCG--CAgUCGCGCAGC- -5' |
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26650 | 3' | -59.9 | NC_005808.1 | + | 27065 | 0.68 | 0.300657 |
Target: 5'- --aUGC-UCAGGGCGUCAGCcccuGCugGUCGa -3' miRNA: 3'- ccgACGcAGUCCCGCAGUCG----CG--CAGC- -5' |
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26650 | 3' | -59.9 | NC_005808.1 | + | 32091 | 0.72 | 0.161919 |
Target: 5'- gGGUUcaaUGUCGuGGGCGUcCAGCGUGUCGa -3' miRNA: 3'- -CCGAc--GCAGU-CCCGCA-GUCGCGCAGC- -5' |
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26650 | 3' | -59.9 | NC_005808.1 | + | 32173 | 0.67 | 0.3639 |
Target: 5'- cGGCaGCGgcCAGGGCGUugagcaacgCGGcCGUGUUGg -3' miRNA: 3'- -CCGaCGCa-GUCCCGCA---------GUC-GCGCAGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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