Results 1 - 11 of 11 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
26650 | 5' | -51.3 | NC_005808.1 | + | 34663 | 0.68 | 0.717883 |
Target: 5'- -aCGGCGUCG-GAcGUGCCCUUG-GCGc -3' miRNA: 3'- aaGUUGCAGUaCU-CGCGGGAAUaCGU- -5' |
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26650 | 5' | -51.3 | NC_005808.1 | + | 475 | 0.69 | 0.664485 |
Target: 5'- -cCGACGaUCAccgccgugcugcuggUGGGCGCCCUgg-GCAu -3' miRNA: 3'- aaGUUGC-AGU---------------ACUCGCGGGAauaCGU- -5' |
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26650 | 5' | -51.3 | NC_005808.1 | + | 6357 | 0.71 | 0.531417 |
Target: 5'- gUCAAUGUCGUGGGCGaCCUgUUcgGCc -3' miRNA: 3'- aAGUUGCAGUACUCGC-GGG-AAuaCGu -5' |
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26650 | 5' | -51.3 | NC_005808.1 | + | 32089 | 0.76 | 0.288501 |
Target: 5'- gUUCAAUGUCGUGGGCGUCCagcGUGUc -3' miRNA: 3'- -AAGUUGCAGUACUCGCGGGaa-UACGu -5' |
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26650 | 5' | -51.3 | NC_005808.1 | + | 7209 | 1.07 | 0.002023 |
Target: 5'- cUUCAACGUCAUGAGCGCCCUUAUGCAa -3' miRNA: 3'- -AAGUUGCAGUACUCGCGGGAAUACGU- -5' |
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26650 | 5' | -51.3 | NC_005808.1 | + | 33179 | 0.66 | 0.825029 |
Target: 5'- ---uGCGgaaGUGGGCGCCCUcg-GCAc -3' miRNA: 3'- aaguUGCag-UACUCGCGGGAauaCGU- -5' |
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26650 | 5' | -51.3 | NC_005808.1 | + | 7523 | 0.66 | 0.825029 |
Target: 5'- uUUCGGCGUCc-GAGUGUUCUUccGCAc -3' miRNA: 3'- -AAGUUGCAGuaCUCGCGGGAAuaCGU- -5' |
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26650 | 5' | -51.3 | NC_005808.1 | + | 28721 | 0.66 | 0.784229 |
Target: 5'- gUCGGCG-CAcgcgaggaUGAGCGCCUUg--GCAu -3' miRNA: 3'- aAGUUGCaGU--------ACUCGCGGGAauaCGU- -5' |
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26650 | 5' | -51.3 | NC_005808.1 | + | 13166 | 0.68 | 0.670348 |
Target: 5'- gUCGGCcaguGUCGgccgcgcUGGGCGCCCUUGgccgGCGu -3' miRNA: 3'- aAGUUG----CAGU-------ACUCGCGGGAAUa---CGU- -5' |
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26650 | 5' | -51.3 | NC_005808.1 | + | 39807 | 0.69 | 0.600941 |
Target: 5'- aUCGACGaaGUGGGCGCaCCUgc-GCAa -3' miRNA: 3'- aAGUUGCagUACUCGCG-GGAauaCGU- -5' |
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26650 | 5' | -51.3 | NC_005808.1 | + | 13765 | 0.71 | 0.4867 |
Target: 5'- -cCAGCGccgaCAUGAGCGCaauCUUGUGCGc -3' miRNA: 3'- aaGUUGCa---GUACUCGCGg--GAAUACGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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