miRNA: 3'- aCga-CGCaaGCGU---CUGGU----------AGCUGCCG- -5'
miRNA: 3'- aCGaCGCa--AGCGUcuGGUAGCuGCCG- -5'
miRNA: 3'- -ACGACGCaAGcGuCUGGUagCUG-CCG- -5'
miRNA: 3'- -ACGACGcaagCGUCuGGUa-GCUGCCG- -5'
miRNA: 3'- -ACG----ACGCa--AGCGU---CUGGUAGCUgc--CG- -5'
Results 61 - 65 of 65 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 26651 | 3' | -55.3 | NC_005808.1 | + | 41454 | 0.66 | 0.658993 |
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Target: 5'- cGacgGCGagCGCAaacGGCCGcugcucaugaUCGACGGCa -3' miRNA: 3'- aCga-CGCaaGCGU---CUGGU----------AGCUGCCG- -5' |
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| 26651 | 3' | -55.3 | NC_005808.1 | + | 28088 | 0.66 | 0.670143 |
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Target: 5'- gGCcGCGgcgUCGUAuuCCGUCG-CGGUg -3' miRNA: 3'- aCGaCGCa--AGCGUcuGGUAGCuGCCG- -5' |
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| 26651 | 3' | -55.3 | NC_005808.1 | + | 12279 | 0.66 | 0.670143 |
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Target: 5'- gUGCUGCG-UC-CcGACCAggGGCaGGCa -3' miRNA: 3'- -ACGACGCaAGcGuCUGGUagCUG-CCG- -5' |
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| 26651 | 3' | -55.3 | NC_005808.1 | + | 11662 | 0.66 | 0.670143 |
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Target: 5'- cUGCUGCccg-GCGG-CCAgcCGGCGGCc -3' miRNA: 3'- -ACGACGcaagCGUCuGGUa-GCUGCCG- -5' |
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| 26651 | 3' | -55.3 | NC_005808.1 | + | 11557 | 0.66 | 0.670143 |
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Target: 5'- cUGCccuuUGCGgugUCGCAggcGACCAUCGAauacGCc -3' miRNA: 3'- -ACG----ACGCa--AGCGU---CUGGUAGCUgc--CG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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